Guiding a confocal microscope by single fluorescent nanoparticles

Hu Cang, C. Shan Xu, Daniel Montiel, Haw Yang

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Confocal optical microscopes offer unparalleled high sensitivity and three-dimensional (3D) imaging capability but require slow point-by-point scanning; they are inefficient for imaging moving objects. We propose a more efficient solution. Instead of indiscriminate scanning, we let the focus of the microscope pursue the object of interest such that no time is wasted on uninformative background, allowing us to visualize 3D trajectories of fluorescent nanoparticles in solution with millisecond temporal and ∼200 nm spatial resolution.

Original languageEnglish (US)
Pages (from-to)2729-2731
Number of pages3
JournalOptics Letters
Volume32
Issue number18
DOIs
StatePublished - Sep 15 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics

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