GuideScan software for improved single and paired CRISPR guide RNA design

Alexendar R. Perez; Yuri Pritykin; Joana A. Vidigal; Sagar Chhangawala; Lee Zamparo; Christina S. Leslie; Andrea Ventura

doi:10.1038/nbt.3804

GuideScan software for improved single and paired CRISPR guide RNA design

Alexendar R. Perez
, Yuri Pritykin
, Joana A. Vidigal
, Sagar Chhangawala
, Lee Zamparo
, Christina S. Leslie
, Andrea Ventura

Research output: Contribution to journal › Article › peer-review

181 Scopus citations

Abstract

We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- A nd paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.

Original language	English (US)
Pages (from-to)	347-349
Number of pages	3
Journal	Nature biotechnology
Volume	35
Issue number	4
DOIs	https://doi.org/10.1038/nbt.3804
State	Published - Apr 1 2017
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

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10.1038/nbt.3804

Cite this

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AU - Leslie, Christina S.

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GuideScan software for improved single and paired CRISPR guide RNA design

Abstract

All Science Journal Classification (ASJC) codes

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