GuideScan software for improved single and paired CRISPR guide RNA design

Alexendar R. Perez, Yuri Pritykin, Joana A. Vidigal, Sagar Chhangawala, Lee Zamparo, Christina S. Leslie, Andrea Ventura

Research output: Contribution to journalArticlepeer-review

149 Scopus citations


We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- A nd paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.

Original languageEnglish (US)
Pages (from-to)347-349
Number of pages3
JournalNature biotechnology
Issue number4
StatePublished - Apr 1 2017
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Bioengineering
  • Molecular Medicine
  • Biotechnology
  • Biomedical Engineering


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