We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- A nd paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.
All Science Journal Classification (ASJC) codes
- Applied Microbiology and Biotechnology
- Molecular Medicine
- Biomedical Engineering