Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing

Xinhuang Kang; Jun Wang; Hong Wu; Ilhan A. Aksay; Jun Liu; Yuehe Lin

doi:10.1016/j.bios.2009.09.004

Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing

Xinhuang Kang, Jun Wang, Hong Wu, Ilhan A. Aksay, Jun Liu, Yuehe Lin

Research output: Contribution to journal › Article › peer-review

1169 Scopus citations

Abstract

Direct electrochemistry of a glucose oxidase (GOD)-graphene-chitosan nanocomposite was studied. The immobilized enzyme retains its bioactivity, exhibits a surface confined, reversible two-proton and two-electron transfer reaction, and has good stability, activity and a fast heterogeneous electron transfer rate with the rate constant (k_s) of 2.83 s^-1. A much higher enzyme loading (1.12 × 10^-9 mol/cm²) is obtained as compared to the bare glass carbon surface. This GOD-graphene-chitosan nanocomposite film can be used for sensitive detection of glucose. The biosensor exhibits a wider linearity range from 0.08 mM to 12 mM glucose with a detection limit of 0.02 mM and much higher sensitivity (37.93 μA mM^-1 cm^-2) as compared with other nanostructured supports. The excellent performance of the biosensor is attributed to large surface-to-volume ratio and high conductivity of graphene, and good biocompatibility of chitosan, which enhances the enzyme absorption and promotes direct electron transfer between redox enzymes and the surface of electrodes.

Original language	English (US)
Pages (from-to)	901-905
Number of pages	5
Journal	Biosensors and Bioelectronics
Volume	25
Issue number	4
DOIs	https://doi.org/10.1016/j.bios.2009.09.004
State	Published - Dec 15 2009

All Science Journal Classification (ASJC) codes

Biophysics
Biotechnology
Biomedical Engineering
Electrochemistry

Keywords

Chitosan
Direct electron transfer
Glucose oxidase
Graphene

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10.1016/j.bios.2009.09.004

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title = "Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing",

abstract = "Direct electrochemistry of a glucose oxidase (GOD)-graphene-chitosan nanocomposite was studied. The immobilized enzyme retains its bioactivity, exhibits a surface confined, reversible two-proton and two-electron transfer reaction, and has good stability, activity and a fast heterogeneous electron transfer rate with the rate constant (ks) of 2.83 s-1. A much higher enzyme loading (1.12 × 10-9 mol/cm2) is obtained as compared to the bare glass carbon surface. This GOD-graphene-chitosan nanocomposite film can be used for sensitive detection of glucose. The biosensor exhibits a wider linearity range from 0.08 mM to 12 mM glucose with a detection limit of 0.02 mM and much higher sensitivity (37.93 μA mM-1 cm-2) as compared with other nanostructured supports. The excellent performance of the biosensor is attributed to large surface-to-volume ratio and high conductivity of graphene, and good biocompatibility of chitosan, which enhances the enzyme absorption and promotes direct electron transfer between redox enzymes and the surface of electrodes.",

keywords = "Chitosan, Direct electron transfer, Glucose oxidase, Graphene",

author = "Xinhuang Kang and Jun Wang and Hong Wu and Aksay, {Ilhan A.} and Jun Liu and Yuehe Lin",

note = "Funding Information: This work was supported by a laboratory-directed research and development program (LDRD) at Pacific Northwest National Laboratory (PNNL). The work was performed at the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the U.S. Department of Energy (DOE) and located at PNNL. PNNL is operated by Battelle for DOE under Contract DE-AC05-76RL01830. IAA acknowledges support from ARO/MURI under grant number W911NF-04-1-0170 .",

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T1 - Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing

AU - Kang, Xinhuang

AU - Wang, Jun

AU - Wu, Hong

AU - Aksay, Ilhan A.

AU - Liu, Jun

AU - Lin, Yuehe

N1 - Funding Information: This work was supported by a laboratory-directed research and development program (LDRD) at Pacific Northwest National Laboratory (PNNL). The work was performed at the Environmental Molecular Sciences Laboratory, a national scientific user facility sponsored by the U.S. Department of Energy (DOE) and located at PNNL. PNNL is operated by Battelle for DOE under Contract DE-AC05-76RL01830. IAA acknowledges support from ARO/MURI under grant number W911NF-04-1-0170 .

PY - 2009/12/15

Y1 - 2009/12/15

N2 - Direct electrochemistry of a glucose oxidase (GOD)-graphene-chitosan nanocomposite was studied. The immobilized enzyme retains its bioactivity, exhibits a surface confined, reversible two-proton and two-electron transfer reaction, and has good stability, activity and a fast heterogeneous electron transfer rate with the rate constant (ks) of 2.83 s-1. A much higher enzyme loading (1.12 × 10-9 mol/cm2) is obtained as compared to the bare glass carbon surface. This GOD-graphene-chitosan nanocomposite film can be used for sensitive detection of glucose. The biosensor exhibits a wider linearity range from 0.08 mM to 12 mM glucose with a detection limit of 0.02 mM and much higher sensitivity (37.93 μA mM-1 cm-2) as compared with other nanostructured supports. The excellent performance of the biosensor is attributed to large surface-to-volume ratio and high conductivity of graphene, and good biocompatibility of chitosan, which enhances the enzyme absorption and promotes direct electron transfer between redox enzymes and the surface of electrodes.

AB - Direct electrochemistry of a glucose oxidase (GOD)-graphene-chitosan nanocomposite was studied. The immobilized enzyme retains its bioactivity, exhibits a surface confined, reversible two-proton and two-electron transfer reaction, and has good stability, activity and a fast heterogeneous electron transfer rate with the rate constant (ks) of 2.83 s-1. A much higher enzyme loading (1.12 × 10-9 mol/cm2) is obtained as compared to the bare glass carbon surface. This GOD-graphene-chitosan nanocomposite film can be used for sensitive detection of glucose. The biosensor exhibits a wider linearity range from 0.08 mM to 12 mM glucose with a detection limit of 0.02 mM and much higher sensitivity (37.93 μA mM-1 cm-2) as compared with other nanostructured supports. The excellent performance of the biosensor is attributed to large surface-to-volume ratio and high conductivity of graphene, and good biocompatibility of chitosan, which enhances the enzyme absorption and promotes direct electron transfer between redox enzymes and the surface of electrodes.

KW - Chitosan

KW - Direct electron transfer

KW - Glucose oxidase

KW - Graphene

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ER -

Glucose Oxidase-graphene-chitosan modified electrode for direct electrochemistry and glucose sensing

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