Glorund interactions in the regulation of gurken and oskar mRNAs

Yossi Kalifa, Stephen T. Armenti, Elizabeth R. Gavis

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Precise temporal and spatial regulation of gene expression during Drosophila oogenesis is essential for patterning the anterior-posterior and dorsal-ventral body axes. Establishment of the anterior-posterior axis requires posterior localization and translational control of both oskar and nanos mRNAs. Establishment of the dorsal-ventral axis depends on the precise restriction of gurken mRNA and protein to the dorsal-anterior corner of the oocyte. We have previously shown that Glorund, the Drosophila hnRNP F/H homolog, contributes to anterior-posterior axis patterning by regulating translation of nanos mRNA, through a direct interaction with its 3′ untranslated region. To investigate the pleiotropy of the glorund mutant phenotype, which includes dorsal-ventral and nuclear morphology defects, we searched for proteins that interact with Glorund. Here we show that Glorund is part of a complex containing the hnRNP protein Hrp48 and the splicing factor Half-pint and plays a role both in mRNA localization and nurse cell chromosome organization, probably by regulating alternative splicing of ovarian tumor. We propose that Glorund is a component of multiple protein complexes and functions both as a translational repressor and splicing regulator for anterior-posterior and dorsal-ventral patterning.

Original languageEnglish (US)
Pages (from-to)68-74
Number of pages7
JournalDevelopmental biology
Volume326
Issue number1
DOIs
StatePublished - Feb 1 2009

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Keywords

  • Glorund
  • Hpf
  • Hrp48
  • Otu
  • Splicing
  • gurken
  • hnRNP
  • mRNA localization
  • oskar

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