@article{84cd45ca5d4d4afdb7bdbec8455277bc,
title = "Global morphogenetic flow is accurately predicted by the spatial distribution of myosin motors",
abstract = "During embryogenesis tissue layers undergo morphogenetic flow rearranging and folding into specific shapes. While developmental biology has identified key genes and local cellular processes, global coordination of tissue remodeling at the organ scale remains unclear. Here, we combine in toto light-sheet microscopy of the Drosophila embryo with quantitative analysis and physical modeling to relate cellular flow with the patterns of force generation during the gastrulation process. We find that the complex spatio-temporal flow pattern can be predicted from the measured meso-scale myosin density and anisotropy using a simple, effective viscous model of the tissue, achieving close to 90% accuracy with one time dependent and two constant parameters. Our analysis uncovers the importance of a) spatial modulation of myosin distribution on the scale of the embryo and b) the non-locality of its effect due to mechanical interaction of cells, demonstrating the need for the global perspective in the study of morphogenetic flow.",
author = "Streichan, {Sebastian J.} and Lefebvre, {Matthew F.} and Nicholas Noll and Wieschaus, {Eric F.} and Shraiman, {Boris I.}",
note = "Funding Information: We thank Trudi Sch{\"u}pbach as well as members of Shraiman and Wieschaus labs for helpful discussions; Lars Hufnagel for initial support with the MuVi SPIM setup; and Reba Samanta for preparing histological samples used to create Figure 1—figure supplement 7. This work was funded by the Gordon and Betty Moore Foundation grant #2919 (SJS), NSF PHY-1220616 (BIS), grant #1K99HD088708-01 from National Institute of Child Health and Human Development (SJS). EFW is an investigator with the Howard Hughes Medical Institute. Funding Information: We thank Trudi Sch{\"u}pbach as well as members of Shraiman and Wieschaus labs for helpful discussions; Lars Hufnagel for initial support with the MuVi SPIM setup; and Reba Samanta for preparing histological samples used to create Figure 1-figure supplement 7. This work was funded by the Gordon and Betty Moore Foundation grant #2919 (SJS), NSF PHY-1220616 (BIS), grant #1K99HD088708-01 from National Institute of Child Health and Human Development (SJS). EFW is an investigator with the Howard Hughes Medical Institute. National Science Foundation PHY-1220616 Boris I Shraiman Howard Hughes Medical Institute Eric F Wieschaus National Institutes of Health NICHD 1K99HD088708 Sebastian J Streichan Gordon and Betty Moore Foundation GBMF #2919 Boris I Shraiman The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Publisher Copyright: {\textcopyright} Streichan et al.",
year = "2018",
month = feb,
day = "9",
doi = "10.7554/eLife.27454",
language = "English (US)",
volume = "7",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}