@inbook{dec9d7a6570f4a80868f1b422fdef4e1,
title = "Global characterization of RNA modifying enzymes with RNA-mediated activity-based protein profiling (RNABPP)",
abstract = "Post-transcriptional RNA modifications can regulate RNA function and play an important role in gene expression. Studying RNA modifying enzymes and their associated modifications remains a considerable challenge. Here we describe the RNA-mediated activity-based protein profiling (RNABPP) methodology, a chemoproteomic strategy for profiling the activity of RNA modifying enzymes in their native context. RNABPP relies upon metabolic RNA labeling with modified ribonucleoside-based probes, combined with protein-RNA enrichment and quantitative proteomics. The RNABPP approach is a general strategy based on chemical reactivity and enzyme mechanism, making it suitable for probing multiple classes of RNA modifying enzymes across diverse biological systems.",
keywords = "activity-based protein profiling, epitranscriptome, modified nucleosides, RNA modifications, RNABPP",
author = "Yu, {Nathan J.} and Jaber, {Qais Z.} and Kleiner, {Ralph E.}",
note = "Publisher Copyright: {\textcopyright} 2024",
year = "2024",
month = jan,
doi = "10.1016/bs.mie.2024.07.010",
language = "English (US)",
isbn = "9780443297243",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "111--125",
editor = "Eichman, {Brandt F.}",
booktitle = "Chemical and Genomic Methods in Nucleic Acid Biology",
address = "United States",
}