Germline P granules are liquid droplets that localize by controlled dissolution/condensation

Clifford P. Brangwynne, Christian R. Eckmann, David S. Courson, Agata Rybarska, Carsten Hoege, Jöbin Gharakhani, Frank Jülicher, Anthony A. Hyman

Research output: Contribution to journalArticle

848 Scopus citations

Abstract

In sexually reproducing organisms, embryos specify germ cells, which ultimately generate sperm and eggs. In Caenorhabditis elegans, the first germ cell is established when RNA and protein-rich P granules localize to the posterior of the one-cell embryo. Localization of P granules and their physical nature remain poorly understood. Here we show that P granules exhibit liquid-like behaviors, including fusion, dripping, and wetting, which we used to estimate their viscosity and surface tension. As with other liquids, P granules rapidly dissolved and condensed. Localization occurred by a biased increase in P granule condensation at the posterior. This process reflects a classic phase transition, in which polarity proteins vary the condensation point across the cell. Such phase transitions may represent a fundamental physicochemical mechanism for structuring the cytoplasm.

Original languageEnglish (US)
Pages (from-to)1729-1732
Number of pages4
JournalScience
Volume324
Issue number5935
DOIs
StatePublished - Jun 26 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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    Brangwynne, C. P., Eckmann, C. R., Courson, D. S., Rybarska, A., Hoege, C., Gharakhani, J., Jülicher, F., & Hyman, A. A. (2009). Germline P granules are liquid droplets that localize by controlled dissolution/condensation. Science, 324(5935), 1729-1732. https://doi.org/10.1126/science.1172046