TY - JOUR
T1 - Germ plasm anchoring is a dynamic state that requires persistent trafficking
AU - Sinsimer, Kristina S.
AU - Lee, Jack J.
AU - Thiberge, Stephan Y.
AU - Gavis, Elizabeth R.
N1 - Funding Information:
We thank S. Little for kindly providing the osk probe, advice on probe preparation and smFISH, and suggesting the nutrient deprivation experiment. We are grateful to T. Chou, A. Ephrussi, P. Lasko, W. Saxton, R. Warrior, M. Welte, E. Wieschaus, and the Bloomington Stock Center for fly stocks. We thank E. Abbaszadeh and B. Bhogal for comments on the manuscript. This work was supported by grants from the National Institutes of Health to K.S.S. (F32GM087005) and E.R.G. (R01GM067758) and the Lewis-Sigler Institute for Integrative Genomics (P50GM071508).
PY - 2013/12/12
Y1 - 2013/12/12
N2 - Localized cytoplasmic determinants packaged as ribonucleoprotein (RNP) particles direct embryonic patterning and cell fate specification in a wide range of organisms. Once established, the asymmetric distributions of such RNP particles must be maintained, often over considerable developmental time. A striking example is the Drosophila germ plasm, which contains RNP particles whose localization to the posterior of the egg during oogenesis results in their asymmetric inheritance and segregation of germline from somatic fates in the embryo. Although actin-based anchoring mechanisms have been implicated, high-resolution live imaging revealed persistent trafficking of germ plasm RNP particles at the posterior cortex of the Drosophila oocyte. This motility relies on cortical microtubules, is mediated by kinesin and dynein motors, and requires coordination between the microtubule and actin cytoskeletons. Finally, we show that RNP particle motility is required for long-term germ plasm retention. We propose that anchoring is a dynamic state that renders asymmetries robust to developmental time and environmental perturbations
AB - Localized cytoplasmic determinants packaged as ribonucleoprotein (RNP) particles direct embryonic patterning and cell fate specification in a wide range of organisms. Once established, the asymmetric distributions of such RNP particles must be maintained, often over considerable developmental time. A striking example is the Drosophila germ plasm, which contains RNP particles whose localization to the posterior of the egg during oogenesis results in their asymmetric inheritance and segregation of germline from somatic fates in the embryo. Although actin-based anchoring mechanisms have been implicated, high-resolution live imaging revealed persistent trafficking of germ plasm RNP particles at the posterior cortex of the Drosophila oocyte. This motility relies on cortical microtubules, is mediated by kinesin and dynein motors, and requires coordination between the microtubule and actin cytoskeletons. Finally, we show that RNP particle motility is required for long-term germ plasm retention. We propose that anchoring is a dynamic state that renders asymmetries robust to developmental time and environmental perturbations
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U2 - 10.1016/j.celrep.2013.10.045
DO - 10.1016/j.celrep.2013.10.045
M3 - Article
C2 - 24290763
AN - SCOPUS:84890177532
SN - 2211-1247
VL - 5
SP - 1169
EP - 1177
JO - Cell Reports
JF - Cell Reports
IS - 5
ER -