Germ-line dependence of the maroon-like maternal effect in Drosophila

J. L. Marsh; E. Wieschaus

doi:10.1016/0012-1606(77)90137-3

Germ-line dependence of the maroon-like maternal effect in Drosophila

J. L. Marsh, E. Wieschaus

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Abstract

We have used pole cell transplantations to construct germ-line mosaics for maroon-like (mal), a maternal effect mutation in Drosophila. Such mosaics allow one to determine the cell type in which a gene is active. We find that the maroon-like maternal effect is (1) autonomous to the germ line and (2) dose sensitive in germ-line mosaics. Aldehyde oxidase activity is used as a histological probe to investigate the tissue and temporal distribution of mal⁺ activity in the developing ovary. The adult ovary shows mal⁺ activity in the germ line at all discernible stages of oogenesis but no activity is observed in the mesodermally derived follicle cells. Differential mal⁺ activity is observed even in the ovary of the third-instar larvae.

Original language	English (US)
Pages (from-to)	396-403
Number of pages	8
Journal	Developmental biology
Volume	60
Issue number	2
DOIs	https://doi.org/10.1016/0012-1606(77)90137-3
State	Published - Oct 15 1977

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology
Developmental Biology

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10.1016/0012-1606(77)90137-3

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@article{96087f27f5a948299855c0863bb37942,

title = "Germ-line dependence of the maroon-like maternal effect in Drosophila",

abstract = "We have used pole cell transplantations to construct germ-line mosaics for maroon-like (mal), a maternal effect mutation in Drosophila. Such mosaics allow one to determine the cell type in which a gene is active. We find that the maroon-like maternal effect is (1) autonomous to the germ line and (2) dose sensitive in germ-line mosaics. Aldehyde oxidase activity is used as a histological probe to investigate the tissue and temporal distribution of mal+ activity in the developing ovary. The adult ovary shows mal+ activity in the germ line at all discernible stages of oogenesis but no activity is observed in the mesodermally derived follicle cells. Differential mal+ activity is observed even in the ovary of the third-instar larvae.",

author = "Marsh, {J. L.} and E. Wieschaus",

note = "Funding Information: This work was supported by a USPHS National Institutes of Health Research Fellowship Award (No. 1F 32 HD05053-01) to J.L.M. The authors would like to thank Dr. W. J. Gehring for his support throughout the course of this work. Dr. Gehring is supported by a Schweizeriche Nationalfonds grant (No. 3.499-0.75). We are also indebted to Ms. Erika Wenger for her excellent secretarial assistance.",

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doi = "10.1016/0012-1606(77)90137-3",

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T1 - Germ-line dependence of the maroon-like maternal effect in Drosophila

AU - Marsh, J. L.

AU - Wieschaus, E.

N1 - Funding Information: This work was supported by a USPHS National Institutes of Health Research Fellowship Award (No. 1F 32 HD05053-01) to J.L.M. The authors would like to thank Dr. W. J. Gehring for his support throughout the course of this work. Dr. Gehring is supported by a Schweizeriche Nationalfonds grant (No. 3.499-0.75). We are also indebted to Ms. Erika Wenger for her excellent secretarial assistance.

PY - 1977/10/15

Y1 - 1977/10/15

N2 - We have used pole cell transplantations to construct germ-line mosaics for maroon-like (mal), a maternal effect mutation in Drosophila. Such mosaics allow one to determine the cell type in which a gene is active. We find that the maroon-like maternal effect is (1) autonomous to the germ line and (2) dose sensitive in germ-line mosaics. Aldehyde oxidase activity is used as a histological probe to investigate the tissue and temporal distribution of mal+ activity in the developing ovary. The adult ovary shows mal+ activity in the germ line at all discernible stages of oogenesis but no activity is observed in the mesodermally derived follicle cells. Differential mal+ activity is observed even in the ovary of the third-instar larvae.

AB - We have used pole cell transplantations to construct germ-line mosaics for maroon-like (mal), a maternal effect mutation in Drosophila. Such mosaics allow one to determine the cell type in which a gene is active. We find that the maroon-like maternal effect is (1) autonomous to the germ line and (2) dose sensitive in germ-line mosaics. Aldehyde oxidase activity is used as a histological probe to investigate the tissue and temporal distribution of mal+ activity in the developing ovary. The adult ovary shows mal+ activity in the germ line at all discernible stages of oogenesis but no activity is observed in the mesodermally derived follicle cells. Differential mal+ activity is observed even in the ovary of the third-instar larvae.

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U2 - 10.1016/0012-1606(77)90137-3

DO - 10.1016/0012-1606(77)90137-3

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VL - 60

SP - 396

EP - 403

JO - Developmental biology

JF - Developmental biology

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ER -

Germ-line dependence of the maroon-like maternal effect in Drosophila

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