Genomic imprinting of Mash2, a mouse gene required for trophoblast development

François Guillemot, Tamara Caspary, Shirley M. Tilghman, Neal G. Copeland, Debra J. Gilbert, Nancy A. Jenkins, David J. Anderson, Alexandra L. Joyner, Janet Rossant, András Nagy

Research output: Contribution to journalArticle

318 Scopus citations

Abstract

The mouse gene Mash2 encodes a transcription factor required for development of trophoblast progenitors. Mash2− homozygous mutant embryos die at 10 days post–coitum from placental failure. Here we show that Mash2 is genomically imprinted. First, Mash2+/− embryos inheriting a wild–type allele from their father die at the same stage as −/− embryos, with a similar placental phenotype. Second, the Mash2 paternal allele is initially expressed by groups of trophoblast cells at 6.5 and 7.5 days post–coitum, but appears almost completely repressed by 8.5 days post–coitum. Finally, we have genetically and physically mapped Mash2 to the distal region of chromosome 7, within a cluster of imprinted genes, including insulin–2, insulin–like growth factor–2 and H19.

Original languageEnglish (US)
Pages (from-to)235-242
Number of pages8
JournalNature Genetics
Volume9
Issue number3
DOIs
StatePublished - Mar 1995

All Science Journal Classification (ASJC) codes

  • Genetics

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    Guillemot, F., Caspary, T., Tilghman, S. M., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Anderson, D. J., Joyner, A. L., Rossant, J., & Nagy, A. (1995). Genomic imprinting of Mash2, a mouse gene required for trophoblast development. Nature Genetics, 9(3), 235-242. https://doi.org/10.1038/ng0395-235