Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts

Janice M. Rossi; David T. Burke; Jimmy C.M. Leung; David S. Koos; Hsiuchen Chen; Shirley M. Tilghman

Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts

Janice M. Rossi, David T. Burke, Jimmy C.M. Leung, David S. Koos, Hsiuchen Chen, Shirley M. Tilghman

Research output: Contribution to journal › Article › peer-review

Abstract

A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.

Original language	English (US)
Pages (from-to)	2456-2460
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	89
Issue number	6
State	Published - 1992

All Science Journal Classification (ASJC) codes

General

Keywords

Chromosome walking
Fu locus
Mouse genetics
Physical mapping

Cite this

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title = "Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts",

abstract = "A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.",

keywords = "Chromosome walking, Fu locus, Mouse genetics, Physical mapping",

author = "Rossi, {Janice M.} and Burke, {David T.} and Leung, {Jimmy C.M.} and Koos, {David S.} and Hsiuchen Chen and Tilghman, {Shirley M.}",

year = "1992",

language = "English (US)",

volume = "89",

pages = "2456--2460",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

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T1 - Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts

AU - Rossi, Janice M.

AU - Burke, David T.

AU - Leung, Jimmy C.M.

AU - Koos, David S.

AU - Chen, Hsiuchen

AU - Tilghman, Shirley M.

PY - 1992

Y1 - 1992

N2 - A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.

AB - A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.

KW - Chromosome walking

KW - Fu locus

KW - Mouse genetics

KW - Physical mapping

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M3 - Article

C2 - 1347950

AN - SCOPUS:0026555663

SN - 0027-8424

VL - 89

SP - 2456

EP - 2460

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 6

ER -

Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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