Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts

J. M. Rossi, D. T. Burke, J. C.M. Leung, D. S. Koos, H. Chen, S. M. Tilghman

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.

Original languageEnglish (US)
Pages (from-to)2456-2460
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number6
DOIs
StatePublished - 1992

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Fu locus
  • chromosome walking
  • mouse genetics
  • physical mapping

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