Genome-wide functional analysis of CREB/Long-term memory-dependent transcription reveals distinct basal and memory gene expression programs

Vanisha Lakhina, Rachel N. Arey, Rachel Kaletsky, Amanda Kauffman, Geneva Stein, William Keyes, Daniel Xu, Coleen T. Murphy

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Induced CREB activity is a hallmark of long-term memory, but the full repertoire of CREB transcriptional targets required specifically for memory is not known in any system. To obtain a more complete picture of the mechanisms involved in memory, we combined memory training with genome-wide transcriptional analysis of C.elegans CREB mutants. This approach identified 757 significant CREB/memory-induced targets and confirmed the involvement of known memory genes from other organisms, but also suggested new mechanisms and novel components that may be conserved through mammals. CREB mediates distinct basal and memory transcriptional programs at least partially through spatial restriction of CREB activity: basal targets are regulated primarily in nonneuronal tissues, while memorytargets are enriched for neuronal expression, emanating from CREB activity in AIM neurons. This suite of novel memory-associated genes will provide a platform for the discovery of orthologous mammalian long-term memory components.

Original languageEnglish (US)
Pages (from-to)330-345
Number of pages16
JournalNeuron
Volume85
Issue number2
DOIs
StatePublished - Jan 21 2015

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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