Genome analysis and detection of a Chilean isolate of Grapevine leafroll associated virus-3

Esteban A. Engel; Cristobal Girardi; Paula F. Escobar; Vania Arredondo; Calixto Domínguez; Tomás Pérez-Acle; Pablo D.T. Valenzuela

doi:10.1007/s11262-008-0241-1

Genome analysis and detection of a Chilean isolate of Grapevine leafroll associated virus-3

Esteban A. Engel, Cristobal Girardi, Paula F. Escobar, Vania Arredondo, Calixto Domínguez, Tomás Pérez-Acle, Pablo D.T. Valenzuela

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The complete genome of the Chilean isolate Cl-766 of Grapevine leafroll-associated virus-3 (GLRaV-3) has been sequenced. This is the first genome sequence obtained from a GLRaV-3 isolate of the Southern hemisphere. The genomic RNA of 17,919 nucleotides contains 13 open reading frames (ORFs) with 5′ and 3′ untranslated regions (UTR) of 158 and 277 nucleotides, respectively. Comparison with NY1, the only isolate with complete genomic sequence available today, shows 97.6% nucleotide identity between the two isolates. Examination of the genome variability shows that most of the genetic diversity is concentrated in ORF1a. Three additional isolates from different geographic regions of Chile were partially sequenced as well, one which showed sequence divergence with respect to the other local and foreign isolates, indicative of different evolutionary constrains. Immunodetection systems were developed using monoclonal and polyclonal antibodies produced against the recombinant major coat protein of GLRaV-3, providing sensitive and specific detection using a triple antibody sandwich-enzyme linked immunosorbent assay (TAS-ELISA) and an immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) assay.

Original language	English (US)
Pages (from-to)	110-118
Number of pages	9
Journal	Virus Genes
Volume	37
Issue number	1
DOIs	https://doi.org/10.1007/s11262-008-0241-1
State	Published - Aug 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics
Molecular Biology
Virology

Keywords

Ampelovirus
Closteroviridae
ELISA
IC-RT-PCR
Sequencing

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10.1007/s11262-008-0241-1

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title = "Genome analysis and detection of a Chilean isolate of Grapevine leafroll associated virus-3",

abstract = "The complete genome of the Chilean isolate Cl-766 of Grapevine leafroll-associated virus-3 (GLRaV-3) has been sequenced. This is the first genome sequence obtained from a GLRaV-3 isolate of the Southern hemisphere. The genomic RNA of 17,919 nucleotides contains 13 open reading frames (ORFs) with 5′ and 3′ untranslated regions (UTR) of 158 and 277 nucleotides, respectively. Comparison with NY1, the only isolate with complete genomic sequence available today, shows 97.6% nucleotide identity between the two isolates. Examination of the genome variability shows that most of the genetic diversity is concentrated in ORF1a. Three additional isolates from different geographic regions of Chile were partially sequenced as well, one which showed sequence divergence with respect to the other local and foreign isolates, indicative of different evolutionary constrains. Immunodetection systems were developed using monoclonal and polyclonal antibodies produced against the recombinant major coat protein of GLRaV-3, providing sensitive and specific detection using a triple antibody sandwich-enzyme linked immunosorbent assay (TAS-ELISA) and an immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) assay.",

keywords = "Ampelovirus, Closteroviridae, ELISA, IC-RT-PCR, Sequencing",

author = "Engel, {Esteban A.} and Cristobal Girardi and Escobar, {Paula F.} and Vania Arredondo and Calixto Dom{\'i}nguez and Tom{\'a}s P{\'e}rez-Acle and Valenzuela, {Pablo D.T.}",

note = "Funding Information: Acknowledgments We thank Professors Nicola Fiore, Patricio Arce, and Juanita Bustamante for plant material, helpful discussions and general support. This research was partially funded by the Chilean Genome Initiative FONDEF G02S1001, UNAB DI-02-06/I, PFB-16/2007, and a Santander-Universia fellowship to EAE.",

year = "2008",

month = aug,

doi = "10.1007/s11262-008-0241-1",

language = "English (US)",

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T1 - Genome analysis and detection of a Chilean isolate of Grapevine leafroll associated virus-3

AU - Engel, Esteban A.

AU - Girardi, Cristobal

AU - Escobar, Paula F.

AU - Arredondo, Vania

AU - Domínguez, Calixto

AU - Pérez-Acle, Tomás

AU - Valenzuela, Pablo D.T.

N1 - Funding Information: Acknowledgments We thank Professors Nicola Fiore, Patricio Arce, and Juanita Bustamante for plant material, helpful discussions and general support. This research was partially funded by the Chilean Genome Initiative FONDEF G02S1001, UNAB DI-02-06/I, PFB-16/2007, and a Santander-Universia fellowship to EAE.

PY - 2008/8

Y1 - 2008/8

N2 - The complete genome of the Chilean isolate Cl-766 of Grapevine leafroll-associated virus-3 (GLRaV-3) has been sequenced. This is the first genome sequence obtained from a GLRaV-3 isolate of the Southern hemisphere. The genomic RNA of 17,919 nucleotides contains 13 open reading frames (ORFs) with 5′ and 3′ untranslated regions (UTR) of 158 and 277 nucleotides, respectively. Comparison with NY1, the only isolate with complete genomic sequence available today, shows 97.6% nucleotide identity between the two isolates. Examination of the genome variability shows that most of the genetic diversity is concentrated in ORF1a. Three additional isolates from different geographic regions of Chile were partially sequenced as well, one which showed sequence divergence with respect to the other local and foreign isolates, indicative of different evolutionary constrains. Immunodetection systems were developed using monoclonal and polyclonal antibodies produced against the recombinant major coat protein of GLRaV-3, providing sensitive and specific detection using a triple antibody sandwich-enzyme linked immunosorbent assay (TAS-ELISA) and an immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) assay.

AB - The complete genome of the Chilean isolate Cl-766 of Grapevine leafroll-associated virus-3 (GLRaV-3) has been sequenced. This is the first genome sequence obtained from a GLRaV-3 isolate of the Southern hemisphere. The genomic RNA of 17,919 nucleotides contains 13 open reading frames (ORFs) with 5′ and 3′ untranslated regions (UTR) of 158 and 277 nucleotides, respectively. Comparison with NY1, the only isolate with complete genomic sequence available today, shows 97.6% nucleotide identity between the two isolates. Examination of the genome variability shows that most of the genetic diversity is concentrated in ORF1a. Three additional isolates from different geographic regions of Chile were partially sequenced as well, one which showed sequence divergence with respect to the other local and foreign isolates, indicative of different evolutionary constrains. Immunodetection systems were developed using monoclonal and polyclonal antibodies produced against the recombinant major coat protein of GLRaV-3, providing sensitive and specific detection using a triple antibody sandwich-enzyme linked immunosorbent assay (TAS-ELISA) and an immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) assay.

KW - Ampelovirus

KW - Closteroviridae

KW - ELISA

KW - IC-RT-PCR

KW - Sequencing

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Genome analysis and detection of a Chilean isolate of Grapevine leafroll associated virus-3

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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