Genome analysis and detection of a Chilean isolate of Grapevine leafroll associated virus-3

Esteban A. Engel, Cristobal Girardi, Paula F. Escobar, Vania Arredondo, Calixto Domínguez, Tomás Pérez-Acle, Pablo D.T. Valenzuela

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The complete genome of the Chilean isolate Cl-766 of Grapevine leafroll-associated virus-3 (GLRaV-3) has been sequenced. This is the first genome sequence obtained from a GLRaV-3 isolate of the Southern hemisphere. The genomic RNA of 17,919 nucleotides contains 13 open reading frames (ORFs) with 5′ and 3′ untranslated regions (UTR) of 158 and 277 nucleotides, respectively. Comparison with NY1, the only isolate with complete genomic sequence available today, shows 97.6% nucleotide identity between the two isolates. Examination of the genome variability shows that most of the genetic diversity is concentrated in ORF1a. Three additional isolates from different geographic regions of Chile were partially sequenced as well, one which showed sequence divergence with respect to the other local and foreign isolates, indicative of different evolutionary constrains. Immunodetection systems were developed using monoclonal and polyclonal antibodies produced against the recombinant major coat protein of GLRaV-3, providing sensitive and specific detection using a triple antibody sandwich-enzyme linked immunosorbent assay (TAS-ELISA) and an immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) assay.

Original languageEnglish (US)
Pages (from-to)110-118
Number of pages9
JournalVirus Genes
Volume37
Issue number1
DOIs
StatePublished - Aug 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Virology

Keywords

  • Ampelovirus
  • Closteroviridae
  • ELISA
  • IC-RT-PCR
  • Sequencing

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    Engel, E. A., Girardi, C., Escobar, P. F., Arredondo, V., Domínguez, C., Pérez-Acle, T., & Valenzuela, P. D. T. (2008). Genome analysis and detection of a Chilean isolate of Grapevine leafroll associated virus-3. Virus Genes, 37(1), 110-118. https://doi.org/10.1007/s11262-008-0241-1