TY - JOUR
T1 - Genetic and molecular analysis of the 87a7 and 87c1 heat-inducible loci of D. melanogaster
AU - Ish-Horowicz, David
AU - Pinchin, Sheena M.
AU - Schedl, Paul
AU - Artavanis-Tsakonas, Spyros
AU - Mirault, Marc Edouard
N1 - Funding Information:
This work was supported by grants from the Swiss National Science Foundation (to W. J. Gehring and A. TissiBres). S. A.-T. was supported by a long-term EMBO fellowship and the Swiss National Science Foundation (grant to W. J. Gehring). P.S. was a postdoctoral fellow of the Helen Hay Whitney Foundation and was also supported by the Roche Research Foundation for Scientific Exchange and Biomedical Collaboration with Switzerland.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1979/12
Y1 - 1979/12
N2 - Two different types of heat-inducible sequences are found at the cytogenetic loci 87A7 and 87C1 of D. melanogaster. One of these codes for the 70,000 dalton heat shock protein (hsp 70) and is found at both loci. The other type of sequence (αβ) codes for an RNA of unknown function and is found only at 87C1. We have completed a study of the organization of the two loci, using deficiencies that delete one or other locus, and have estimated the number of the hsp 70 genes at each locus. Thus in at least three strains of flies there are a total of five coding sequences, three at 87C1 and two at 87A7. Restriction mapping of the coding regions at the two loci reveals that each of the two cytogenetic loci has its own characteristic coding sequence. The overall organization of the two loci appears to differ considerably. The αβ and hsp 70 heat-induced sequences at 87C1 are closely linked and are contained within two Eco RI restriction fragments.
AB - Two different types of heat-inducible sequences are found at the cytogenetic loci 87A7 and 87C1 of D. melanogaster. One of these codes for the 70,000 dalton heat shock protein (hsp 70) and is found at both loci. The other type of sequence (αβ) codes for an RNA of unknown function and is found only at 87C1. We have completed a study of the organization of the two loci, using deficiencies that delete one or other locus, and have estimated the number of the hsp 70 genes at each locus. Thus in at least three strains of flies there are a total of five coding sequences, three at 87C1 and two at 87A7. Restriction mapping of the coding regions at the two loci reveals that each of the two cytogenetic loci has its own characteristic coding sequence. The overall organization of the two loci appears to differ considerably. The αβ and hsp 70 heat-induced sequences at 87C1 are closely linked and are contained within two Eco RI restriction fragments.
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U2 - 10.1016/0092-8674(79)90245-9
DO - 10.1016/0092-8674(79)90245-9
M3 - Article
C2 - 117906
AN - SCOPUS:0018575254
SN - 0092-8674
VL - 18
SP - 1351
EP - 1358
JO - Cell
JF - Cell
IS - 4
ER -