Genetic analysis of response regulator activation in bacterial chemotaxis suggests an intermolecular mechanism

Sandra Da Re, Tatiana Tolstykh, Peter M. Wolanin, Jeffry B. Stock

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Response regulator proteins of two-component systems are usually activated by phosphorylation. The phosphorylated response regulator protein CheY-P mediates the chemotaxis response in Escherichia coli. We performed random mutagenesis and selected CheY mutants that are constitutively active in the absence of phosphorylation. Although a single amino acid substitution can lead to constitutive activation, no single DNA base change can effect such a transition. Numerous different sets of mutations that activate in synergy were selected in several different combinations. These mutations were all located on the side of CheY defined by α4, β5, α5, and α1. Our findings argue against the two-state hypothesis for response regulator activation. We propose an alternative intermolecular mechanism that involves a dynamic interplay between response regulators and their effector targets.

Original languageEnglish (US)
Pages (from-to)2644-2654
Number of pages11
JournalProtein Science
Volume11
Issue number11
DOIs
StatePublished - Nov 2002

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Keywords

  • CheY
  • Chemotaxis
  • Escherichia coli
  • Random mutagenesis
  • Two-component systems

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