Genes for the 70,000 dalton heat shock protein in two cloned D. melanogaster DNA segments

Spyros Artavanis-Tsakonas, Paul Schedl, Marc Edouard Mirault, Larry Moran, John Lis

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Two hybrid plasmids, 56H8 and 132E3, which contain D. melanogaster (Dm) DNA segments coding for the 70,000 dalton heat shock protein, have been isolated(Schedl et al., 1978). The analysis of the sequence arrangement presented in the accompanying paper (Moran et al., 1979) shows that both Dm segments contain homologous regions composed of three distinct sequence elements which together define a common unit. We report here that the sequences complementary to the 70,000 dalton protein mRNA appear to be confined to a major portion of the largest element of the common unit and that the other sequence elements are located at the 5′ end of the gene. We have also determined the cytogenetic location of the 70,000 dalton protein genes and have investigated whether these sites are transcriptionally active in salivary gland chromosomes.

Original languageEnglish (US)
Pages (from-to)9-18
Number of pages10
JournalCell
Volume17
Issue number1
DOIs
StatePublished - May 1979
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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