TY - JOUR
T1 - Genes for the 70,000 dalton heat shock protein in two cloned D. melanogaster DNA segments
AU - Artavanis-Tsakonas, Spyros
AU - Schedl, Paul
AU - Mirault, Marc Edouard
AU - Moran, Larry
AU - Lis, John
N1 - Funding Information:
This work was supported by grants from the Swiss National Science Foundation (to W.J. Gehring and A. Tissieres). S.A.-T. was supported by a long-term EMBO fellowship and the Swiss National Science Foundation (grant to W.J.G.). P.S. and J.L. were postdoctoral fellows of the Helen Hay Whitney Foundation. P.S. was also supported by the Roche Research Foundation for Scientific Exchange and Biomedical Collaboration with Switzerland. We thank M. Schedl. G. Martin, M. Stamper and M. Stempfel for their valuable assistance: Dr. W. Bender for a gift of dBrU-tailed trypanosome DNA; 0. Jenni for drawings and plates: W. J. Gehring, D. Hogness and A. Tissieres for advice and encouragement: and Dr. Jean-David Rochaix for his comments on the manuscript.
PY - 1979/5
Y1 - 1979/5
N2 - Two hybrid plasmids, 56H8 and 132E3, which contain D. melanogaster (Dm) DNA segments coding for the 70,000 dalton heat shock protein, have been isolated(Schedl et al., 1978). The analysis of the sequence arrangement presented in the accompanying paper (Moran et al., 1979) shows that both Dm segments contain homologous regions composed of three distinct sequence elements which together define a common unit. We report here that the sequences complementary to the 70,000 dalton protein mRNA appear to be confined to a major portion of the largest element of the common unit and that the other sequence elements are located at the 5′ end of the gene. We have also determined the cytogenetic location of the 70,000 dalton protein genes and have investigated whether these sites are transcriptionally active in salivary gland chromosomes.
AB - Two hybrid plasmids, 56H8 and 132E3, which contain D. melanogaster (Dm) DNA segments coding for the 70,000 dalton heat shock protein, have been isolated(Schedl et al., 1978). The analysis of the sequence arrangement presented in the accompanying paper (Moran et al., 1979) shows that both Dm segments contain homologous regions composed of three distinct sequence elements which together define a common unit. We report here that the sequences complementary to the 70,000 dalton protein mRNA appear to be confined to a major portion of the largest element of the common unit and that the other sequence elements are located at the 5′ end of the gene. We have also determined the cytogenetic location of the 70,000 dalton protein genes and have investigated whether these sites are transcriptionally active in salivary gland chromosomes.
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U2 - 10.1016/0092-8674(79)90290-3
DO - 10.1016/0092-8674(79)90290-3
M3 - Article
C2 - 110461
AN - SCOPUS:0018366866
SN - 0092-8674
VL - 17
SP - 9
EP - 18
JO - Cell
JF - Cell
IS - 1
ER -