@inbook{915448e48d9f4a9c8e2e7a800888a4c9,
title = "Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos",
abstract = "Large fragment knock-in mouse models such as reporters and conditional mutant mice are important models for biological research. Here we describe 2-cell (2C)-homologous recombination (HR)-CRISPR, a highly efficient method to generate large fragment knock-in mouse models by CRISPR-based genome engineering. Using this method, knock-in founders can be generated routinely in a time frame of about two months with high germline transmission efficiency. 2C-HR-CRISPR will significantly promote the advancement of basic and translational research using genetic mouse models.",
keywords = "2-Cell stage, CRISPR-Cas9, Homologous recombination, Knock-in",
author = "Bin Gu and Marina Gertsenstein and Eszter Posfai",
note = "Publisher Copyright: {\textcopyright} 2020, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2020",
doi = "10.1007/978-1-4939-9837-1_7",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "89--100",
booktitle = "Methods in Molecular Biology",
}