Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos

Bin Gu, Marina Gertsenstein, Eszter Posfai

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Large fragment knock-in mouse models such as reporters and conditional mutant mice are important models for biological research. Here we describe 2-cell (2C)-homologous recombination (HR)-CRISPR, a highly efficient method to generate large fragment knock-in mouse models by CRISPR-based genome engineering. Using this method, knock-in founders can be generated routinely in a time frame of about two months with high germline transmission efficiency. 2C-HR-CRISPR will significantly promote the advancement of basic and translational research using genetic mouse models.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages89-100
Number of pages12
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2066
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • 2-Cell stage
  • CRISPR-Cas9
  • Homologous recombination
  • Knock-in

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  • Cite this

    Gu, B., Gertsenstein, M., & Posfai, E. (2020). Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos. In Methods in Molecular Biology (pp. 89-100). (Methods in Molecular Biology; Vol. 2066). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9837-1_7