Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing

Sine L. Svenningsen, Kimberly C. Tu, Bonnie L. Bassler

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Quorum sensing is a mechanism of cell-to-cell communication that allows bacteria to coordinately regulate gene expression in response to changes in cell-population density. At the core of the Vibrio cholerae quorum-sensing signal transduction pathway reside four homologous small RNAs (sRNAs), named the quorum regulatory RNAs 1-4 (Qrr1-4). The four Qrr sRNAs are functionally redundant. That is, expression of any one of them is sufficient for wild-type quorum-sensing behaviour. Here, we show that the combined action of two feedback loops, one involving the sRNA-activator LuxO and one involving the sRNA-target HapR, promotes gene dosage compensation between the four qrr genes. Gene dosage compensation adjusts the total Qrr1-4 sRNA pool and provides the molecular mechanism underlying sRNA redundancy. The dosage compensation mechanism is exquisitely sensitive to small perturbations in Qrr levels. Precisely maintained Qrr levels are required to direct the proper timing and correct patterns of expression of quorum-sensing-regulated target genes.

Original languageEnglish (US)
Pages (from-to)429-439
Number of pages11
JournalEMBO Journal
Volume28
Issue number4
DOIs
StatePublished - Feb 18 2009

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Keywords

  • Dosage compensation
  • Pathogenesis
  • Quorum sensing
  • sRNA

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