@article{46fb7e1cd62f4d44bd5120321ac17327,
title = "Gdf3 is required for robust nodal signaling during germ layer formation and left-right patterning",
abstract = "Vertebrate embryonic patterning depends on signaling from Nodal, a TGFb superfamily member. There are three Nodal orthologs in zebrafish; southpaw directs left-right asymmetries, while squint and cyclops function earlier to pattern mesendoderm. TGFb member Vg1 is implicated in mesoderm formation but the role of the zebrafish ortholog, Growth differentiation factor 3 (Gdf3), has not been fully explored. We show that zygotic expression of gdf3 is dispensable for embryonic development, while maternally deposited gdf3 is required for mesendoderm formation and dorsal-ventral patterning. We further show that Gdf3 can affect left- right patterning at multiple stages, including proper development of regional cell morphology in Kupffer{\textquoteright}s vesicle and the establishment of southpaw expression in the lateral plate mesoderm. Collectively, our data indicate that gdf3 is critical for robust Nodal signaling at multiple stages in zebrafish embryonic development.",
author = "Pelliccia, {Jose L.} and Jindal, {Granton A.} and Burdine, {Rebecca D.}",
note = "Funding Information: The content of this manuscript is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health or National Science Foundation. Funding Information: We are grateful for the exemplary collegiality of Alex Schier and Joe Yost in coordinating publications. We thank Victoria L Patterson and Daniel T Grimes for the constructive comments, helpful advice, and assistance in editing the manuscript. We thank Philip Johnson for zebrafish care, and Dr. Gary Laevsky and the Molecular Biology Confocal Microscopy Facility, which is a Nikon Center of Excellence, for microscopy support. GAJ acknowledges support from National Science Foundation Graduate Research Fellowship Grant DGE 1148900. This work was supported by funding awarded to RDB from the National Institute of Child Health and Development, grant R01HD048584. The content of this manuscript is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Funding Information: National Science Foundation Publisher Copyright: {\textcopyright} Pelliccia et al.",
year = "2017",
month = nov,
day = "15",
doi = "10.7554/eLife.28635",
language = "English (US)",
volume = "6",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}