Gap junctions in the ventral hippocampal-medial prefrontal pathway are involved in anxiety regulation

Timothy J. Schoenfeld, Alexander D. Kloth, Brian Hsueh, Matthew B. Runkle, Gary A. Kane, Samuel S.H. Wang, Elizabeth Gould

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Anxiety disorders are highly prevalent but little is known about their underlying mechanisms. Gap junctions exist in brain regions important for anxiety regulation, such as the ventral hippocampus (vHIP) and mPFC, but their functions in these areas have not been investigated. Using pharmacological blockade of neuronal gap junctions combined with electrophysiological recordings, we found that gap junctions play a role in theta rhythm in the vHIP and mPFC of adult mice. Bilateral infusion of neuronal gap junction blockers into the vHIP decreased anxiety-like behavior on the elevated plus maze and open field. Similar anxiolytic effects were observed with unilateral infusion of these drugs into the vHIP combined with contralateral infusion into the mPFC. No change in anxious behavior was observed with gap junction blockade in the unilateral vHIP alone or in the bilateral dorsal HIP. Since physical exercise is known to reduce anxiety, we examined the effects of long-term running on the expression of the neuronal gap junction protein connexin-36 among inhibitory interneurons and found a reduction in the vHIP. Despite this change, we observed no alteration in theta frequency or power in long-term runners. Collectively, these findings suggest that neuronal gap junctions in the vHIP-mPFC pathway are important for theta rhythm and anxiety regulation under sedentary conditions but that additional mechanisms are likely involved in running-induced reduction in anxiety.

Original languageEnglish (US)
Pages (from-to)15679-15688
Number of pages10
JournalJournal of Neuroscience
Volume34
Issue number47
DOIs
StatePublished - Nov 19 2014

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Keywords

  • Anxiety
  • Connexin
  • Gap junctions
  • Medial prefrontal cortex
  • Theta rhythm
  • Ventral hippocampus

Fingerprint Dive into the research topics of 'Gap junctions in the ventral hippocampal-medial prefrontal pathway are involved in anxiety regulation'. Together they form a unique fingerprint.

  • Cite this