GAGA mediates the enhancer blocking activity of the eve promoter in the Drosophila embryo

Sumio Ohtsuki, Michael Levine

Research output: Contribution to journalArticlepeer-review

118 Scopus citations

Abstract

Insulator DNAs and promoter competition regulate enhancer-promoter interactions within complex genetic loci. A transgenic embryo assay was used to obtain evidence that the Drosophila eve promoter possesses an insulator activity that can be uncoupled from the core elements that mediate competition. The eve promoter contains an optimal TATA element and a GAGA sequence. The analysis of various chimeric promoters provides evidence that TATA is essential for promoter competition, whereas GAGA mediates enhancer blocking. The Trithorax-like (Trl) protein interacts with GAGA, and mutations in trl attenuate eve promoter insulator activity. We suggest that Trl-GAGA increases the stability of enhancer-promoter interactions by creating an open chromatin configuration at the core promoter.

Original languageEnglish (US)
Pages (from-to)3325-3330
Number of pages6
JournalGenes and Development
Volume12
Issue number21
DOIs
StatePublished - Nov 1 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Keywords

  • Drosophila embryo
  • Enhancer-promoter interactions
  • GAGA
  • Insulators

Fingerprint Dive into the research topics of 'GAGA mediates the enhancer blocking activity of the eve promoter in the Drosophila embryo'. Together they form a unique fingerprint.

Cite this