Abstract
Pseudorabies virus encodes a membrane protein (Us9) that is essential for the axonal sorting of virus particles within neurons and anterograde spread in the mammalian nervous system. Enhanced green fluorescent protein (GFP)-tagged Us9 mimicked the trafficking properties of the wild-type protein in nonneuronal cells. We constructed a pseudorabies virus strain that expressed Us9-GFP and tested its spread capabilities in the rat visual system and in primary neuronal cultures. We report that Us9-EGFP does not promote anterograde spread of infection and may disrupt packing of viral membrane proteins in lipid rafts, an essential step for Us9-mediated axonal sorting.
Original language | English (US) |
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Pages (from-to) | 10308-10311 |
Number of pages | 4 |
Journal | Journal of virology |
Volume | 82 |
Issue number | 20 |
DOIs | |
State | Published - Oct 2008 |
All Science Journal Classification (ASJC) codes
- Insect Science
- Virology
- Microbiology
- Immunology