Functional analysis of point mutations in the AAUAAA motif of the SV40 late polyadenylation signal

Jeffrey Wilus, Stefan M. Pettine, Thomas Shenk

Research output: Contribution to journalArticle

61 Scopus citations

Abstract

We have constructed 14 independent point mutations in the conserved AAUAAA element of the SV40 late polyadenylation signal in order to study the recognition and function of alternative polyadenylation signals. A variant RNA containing an AUUAAA was polyadenylated at 20% the level of wild-type substrate RNA, while all other derivatives tested were not functional In vicro. The AUUAAA variant RNA formed specific complexes in native polyacrylamide gels and crosslinked to the AAUAAA-specific 64kd polypeptide, but at a lower efficiency than wild-type substrate RNA.

Original languageEnglish (US)
Pages (from-to)3899-3908
Number of pages10
JournalNucleic acids research
Volume17
Issue number10
DOIs
StatePublished - May 25 1989

All Science Journal Classification (ASJC) codes

  • Genetics

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