Formation of a highly peptide-receptive state of class II MHC

Joshua D. Rabinowitz, Marija Vrljic, Peter M. Kasson, Michael N. Liang, Robert Busch, J. Jay Boniface, Mark M. Davis, Harden M. McConnell

Research output: Contribution to journalArticlepeer-review

116 Scopus citations

Abstract

Peptide binding to class II MHC proteins occurs in acidic endosomal compartments following dissociation of class II-associated invariant chain peptide (CLIP). Based on peptide binding both to empty class II MHC and to molecules preloaded with peptides including CLIP, we find evidence for two isomeric forms of empty MHC. One (inactive) does not bind peptide. The other (active) binds peptide rapidly, with k(on) 1000 fold faster than previous estimates. The active isomer can be formed either by slow isomerization of the inactive molecule or by dissociation of a preformed peptide/MHC complex. In the absence of peptide, the active isomer is unstable, rapidly converting to the inactive isomer. These results demonstrate that fast peptide binding is an inherent property of one isomer of empty class II MHC. Dissociation of peptides such as CLIP yields this transient, peptide-receptive isomer.

Original languageEnglish (US)
Pages (from-to)699-709
Number of pages11
JournalImmunity
Volume9
Issue number5
DOIs
StatePublished - Nov 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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