Fluorescent monitoring of kinase activity in real time: Development of a robust fluorescence-based assay for Abl tyrosine kinase activity

Roseanne M. Hofmann, Graham J. Cotton, Emmanuel J. Chang, Ephraim Vidal, Darren Veach, William Bornmann, Tom W. Muir

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Fluorescent biosensors hold great promise for drug discovery. Using a solid-phase version of protein semi-synthesis, we incorporated two fluorophores at specific sites within a truncated version of the c-Crk-II protein. The resulting fluorescent protein biosensor permits the real-time monitoring of Abl kinase activity and provides a robust and rapid method for assaying Abl kinase inhibitors.

Original languageEnglish (US)
Pages (from-to)3091-3094
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume11
Issue number24
DOIs
StatePublished - Dec 17 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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