@article{887b2f9bf10742509a119a56ee37286b,
title = "Fluctuations in populations of subsurface methane oxidizers in coordination with changes in electron acceptor availability",
abstract = "The concentrations of electron donors and acceptors in the terrestrial subsurface biosphere fluctuate due to migration and mixing of subsurface fluids, but the mechanisms and rates at which microbial communities respond to these changes are largely unknown. Subsurface microbial communities exhibit long cellular turnover times and are often considered relatively static-generating just enough ATP for cellular maintenance. Here, we investigated how subsurface populations of CH4 oxidizers respond to changes in electron acceptor availability by monitoring the biological and geochemical composition in a 1339 m-below-land-surface (mbls) fluid-filled fracture over the course of both longer (2.5 year) and shorter (2-week) time scales. Using a combination of metagenomic, metatranscriptomic, and metaproteomic analyses, we observe that the CH4 oxidizers within the subsurface microbial community change in coordination with electron acceptor availability over time. We then validate these findings through a series of 13C-CH4 laboratory incubation experiments, highlighting a connection between composition of subsurface CH4 oxidizing communities and electron acceptor availability.",
keywords = "Archaea, Metagenomics, Methane oxidation, Subsurface",
author = "C. Magnabosco and Timmers, {P. H.A.} and Lau, {M. C.Y.} and G. Borgonie and B. Linage-Alvarez and O. Kuloyo and R. Alleva and Kieft, {T. L.} and Slater, {G. F.} and {van Heerden}, E. and {Sherwood Lollar}, B. and Onstott, {Tullis C.}",
note = "Funding Information: This research was supported by funding from the National Science Foundation to T.C.O. (EAR-0948659) and to TLK (EAR-0948335) and the Deep Carbon Observatory (Alfred P. Sloan Foundation) to M.C.Y.L. (Sloan 2013-10-03, subaward 48045). Research of P.H.A.T. is supported by the Soehngen Institute of Anaerobic Microbiology (SIAM) Gravitation grant (024.002.002) of the Netherlands Ministry of Education, Culture and Science and the Netherlands Organisation for Scientific Research (NWO). Partial support for isotopic analyses was provided by the Natural Sciences and Engineering Research Council of Canada. Funding Information: We are indebted to the logistical support of Sibanye Gold Limited, the management and staff of Beatrix Gold Mine and specifically to S. Maphanga of Beatrix gold mine. We thank Matthew Cahn (Department of Molecular Biology, Princeton University) and the staff of Research Computing (Office of Information Technology, Princeton University), especially Robert Knight, for their technical support with the computational analyses. This research was supported by funding from the National Science Foundation to T.C.O. (EAR-0948659) and to TLK (EAR-0948335) and the Deep Carbon Observatory (Alfred P. Sloan Foundation) to M.C.Y.L. (Sloan 2013-10-03, subaward 48045). Research of P.H.A.T. is supported by the Soehngen Institute of Anaerobic Microbiology (SIAM) Gravitation grant (024.002.002) of the Netherlands Ministry of Education, Culture and Science and the Netherlands Organisation for Scientific Research (NWO). Partial support for isotopic analyses was provided by the Natural Sciences and Engineering Research Council of Canada Publisher Copyright: {\textcopyright} FEMS 2018.",
year = "2018",
month = jul,
day = "1",
doi = "10.1093/femsec/fiy089",
language = "English (US)",
volume = "94",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Wiley-Blackwell",
number = "7",
}