Flow-scanning optical tomography

Nicolas C. Pégard, Marton L. Toth, Monica Driscoll, Jason W. Fleischer

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We present a 3D tomography technique for in vivo observation of microscopic samples. The method combines flow in a microfluidic channel, illumination through a slit aperture, and a Fourier lens for simultaneous acquisition of multiple perspective angles in the phase-space domain. The technique is non-invasive and naturally robust to parasitic sample motion. 3D absorption is retrieved using standard back-projection algorithms, here a limited-domain inverse radon transform. Simultaneously, 3D differential phase contrast images are obtained by computational refocusing and comparison of complementary illumination angles. We implement the technique on a modified glass slide which can be mounted directly on existing optical microscopes. We demonstrate both amplitude and phase tomography on live, freely swimming C. elegans nematodes. This journal is

Original languageEnglish (US)
Pages (from-to)4447-4450
Number of pages4
JournalLab on a Chip
Volume14
Issue number23
DOIs
StatePublished - Dec 7 2014

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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    Pégard, N. C., Toth, M. L., Driscoll, M., & Fleischer, J. W. (2014). Flow-scanning optical tomography. Lab on a Chip, 14(23), 4447-4450. https://doi.org/10.1039/c4lc00701h