Flow-scanning optical tomography

Nicolas C. Pégard, Marton L. Toth, Monica Driscoll, Jason W. Fleischer

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


We present a 3D tomography technique for in vivo observation of microscopic samples. The method combines flow in a microfluidic channel, illumination through a slit aperture, and a Fourier lens for simultaneous acquisition of multiple perspective angles in the phase-space domain. The technique is non-invasive and naturally robust to parasitic sample motion. 3D absorption is retrieved using standard back-projection algorithms, here a limited-domain inverse radon transform. Simultaneously, 3D differential phase contrast images are obtained by computational refocusing and comparison of complementary illumination angles. We implement the technique on a modified glass slide which can be mounted directly on existing optical microscopes. We demonstrate both amplitude and phase tomography on live, freely swimming C. elegans nematodes.

Original languageEnglish (US)
Pages (from-to)4447-4450
Number of pages4
JournalLab on a Chip
Issue number23
StatePublished - Dec 7 2014

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Bioengineering
  • Biochemistry
  • Biomedical Engineering


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