We apply the principle of structured illumination microscopy to a fluidic imaging device. The necessary phase shifts are no longer obtained by controlled displacement of the illumination pattern but by flowing the sample itself. The resulting scheme retains all the benefits of fluid systems while enabling easy integration with existing microscopes, flow cytometers, and aquatic imagers. We present the theory of flow-based structured illumination and demonstrate the technique experimentally by reconstructing super-resolved images of yeast cells.
All Science Journal Classification (ASJC) codes
- Physics and Astronomy (miscellaneous)