Flow-based structured illumination

Chien Hung Lu; Nicolas C. Pégard; Jason W. Fleischer

doi:10.1063/1.4802091

Flow-based structured illumination

Chien Hung Lu, Nicolas C. Pégard, Jason W. Fleischer

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

We apply the principle of structured illumination microscopy to a fluidic imaging device. The necessary phase shifts are no longer obtained by controlled displacement of the illumination pattern but by flowing the sample itself. The resulting scheme retains all the benefits of fluid systems while enabling easy integration with existing microscopes, flow cytometers, and aquatic imagers. We present the theory of flow-based structured illumination and demonstrate the technique experimentally by reconstructing super-resolved images of yeast cells.

Original language	English (US)
Article number	161115
Journal	Applied Physics Letters
Volume	102
Issue number	16
DOIs	https://doi.org/10.1063/1.4802091
State	Published - Apr 22 2013

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

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10.1063/1.4802091

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author = "Lu, {Chien Hung} and P{\'e}gard, {Nicolas C.} and Fleischer, {Jason W.}",

note = "Funding Information: The authors thank Professor Howard A. Stone and the Complex Fluid Group for useful discussions. This work was supported by the AFOSR.",

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AU - Pégard, Nicolas C.

AU - Fleischer, Jason W.

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Y1 - 2013/4/22

N2 - We apply the principle of structured illumination microscopy to a fluidic imaging device. The necessary phase shifts are no longer obtained by controlled displacement of the illumination pattern but by flowing the sample itself. The resulting scheme retains all the benefits of fluid systems while enabling easy integration with existing microscopes, flow cytometers, and aquatic imagers. We present the theory of flow-based structured illumination and demonstrate the technique experimentally by reconstructing super-resolved images of yeast cells.

AB - We apply the principle of structured illumination microscopy to a fluidic imaging device. The necessary phase shifts are no longer obtained by controlled displacement of the illumination pattern but by flowing the sample itself. The resulting scheme retains all the benefits of fluid systems while enabling easy integration with existing microscopes, flow cytometers, and aquatic imagers. We present the theory of flow-based structured illumination and demonstrate the technique experimentally by reconstructing super-resolved images of yeast cells.

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Flow-based structured illumination

Abstract

All Science Journal Classification (ASJC) codes

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