Flow-based structured illumination

Chien Hung Lu, Nicolas C. Pégard, Jason W. Fleischer

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We apply the principle of structured illumination microscopy to a fluidic imaging device. The necessary phase shifts are no longer obtained by controlled displacement of the illumination pattern but by flowing the sample itself. The resulting scheme retains all the benefits of fluid systems while enabling easy integration with existing microscopes, flow cytometers, and aquatic imagers. We present the theory of flow-based structured illumination and demonstrate the technique experimentally by reconstructing super-resolved images of yeast cells.

Original languageEnglish (US)
Article number161115
JournalApplied Physics Letters
Volume102
Issue number16
DOIs
StatePublished - Apr 22 2013

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

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