Fixed and live visualization of RNAs in Drosophila oocytes and embryos

Evan K. Abbaszadeh; Elizabeth R. Gavis

doi:10.1016/j.ymeth.2016.01.018

Fixed and live visualization of RNAs in Drosophila oocytes and embryos

Evan K. Abbaszadeh, Elizabeth R. Gavis

Molecular Biology

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The ability to visualize RNA in situ is essential to dissect mechanisms for the temporal and spatial regulation of gene expression that drives development. Although considerable attention has been focused on transcriptional control, studies in model organisms like Drosophila have highlighted the importance of post-transcriptional mechanisms - most notably intracellular mRNA localization - in the formation and patterning of the body axes, specification of cell fates, and polarized cell functions. Our understanding of both types of regulation has been greatly advanced by technological innovations that enable a combination of highly quantitative and dynamic analysis of RNA. This review presents two methods, single molecule fluorescence in situ hybridization for high resolution quantitative RNA detection in fixed Drosophila oocytes and embryos and genetically encoded fluorescent RNA labeling for detection in live cells.

Original language	English (US)
Pages (from-to)	34-41
Number of pages	8
Journal	Methods
Volume	98
DOIs	https://doi.org/10.1016/j.ymeth.2016.01.018
State	Published - Apr 1 2016

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology
Molecular Biology

Keywords

In vivo RNA labeling
Intracellular mRNA localization
MS2/MCP
PP7/PCP
mRNA expression patterns
smFISH

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10.1016/j.ymeth.2016.01.018

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abstract = "The ability to visualize RNA in situ is essential to dissect mechanisms for the temporal and spatial regulation of gene expression that drives development. Although considerable attention has been focused on transcriptional control, studies in model organisms like Drosophila have highlighted the importance of post-transcriptional mechanisms - most notably intracellular mRNA localization - in the formation and patterning of the body axes, specification of cell fates, and polarized cell functions. Our understanding of both types of regulation has been greatly advanced by technological innovations that enable a combination of highly quantitative and dynamic analysis of RNA. This review presents two methods, single molecule fluorescence in situ hybridization for high resolution quantitative RNA detection in fixed Drosophila oocytes and embryos and genetically encoded fluorescent RNA labeling for detection in live cells.",

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Fixed and live visualization of RNAs in Drosophila oocytes and embryos

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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