@article{deab30a09fa9444fbf0490bd68a421f7,
title = "Field-deployable viral diagnostics using CRISPR-Cas13",
abstract = "Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations as low as 1 copy per microliter. We developed HUDSON (heating unextracted diagnostic samples to obliterate nucleases), a protocol that pairs with SHERLOCK for viral detection directly from bodily fluids, enabling instrument-free DENV detection directly from patient samples in <2 hours. We further demonstrate that SHERLOCK can distinguish the four DENV serotypes, as well as region-specific strains of ZIKV from the 2015–2016 pandemic. Finally, we report the rapid (<1 week) design and testing of instrument-free assays to detect clinically relevant viral single-nucleotide polymorphisms.",
author = "Cameron Myhrvold and Freije, {Catherine A.} and Gootenberg, {Jonathan S.} and Abudayyeh, {Omar O.} and Metsky, {Hayden C.} and Durbin, {Ann F.} and Kellner, {Max J.} and Tan, {Amanda L.} and Paul, {Lauren M.} and Parham, {Leda A.} and Garcia, {Kimberly F.} and Barnes, {Kayla G.} and Bridget Chak and Adriano Mondini and Nogueira, {Mauricio L.} and Sharon Isern and Michael, {Scott F.} and Ivette Lorenzana and Yozwiak, {Nathan L.} and MacInnis, {Bronwyn L.} and Irene Bosch and Lee Gehrke and Feng Zhang and Sabeti, {Pardis C.}",
note = "Funding Information: We thank S. Schaffner, A. Lin, and other Sabeti lab members for useful feedback; J. Strecker for providing SUMO protease; and the Florida Department of Health, Miami-Dade County Mosquito Control, and Boca Biolistics for support with patient and mosquito samples. Funding: We acknowledge funding from HHMI, the Broad Institute Chemical Biology and Therapeutics Science Shark Tank, NIH grant U19AI110818, and Defense Advanced Research Projects Agency grant D18AC00006. The views, opinions, and/or findings expressed should not be interpreted as representing the official views or policies of the Department of Defense or the U.S. government. This study has been approved for public release; distribution is unlimited. C.M. is supported by HHMI. O.O.A. is supported by a Paul and Daisy Soros fellowship and NIH grant F30 NRSA 1F30-CA210382. S.I. and S.F.M. are supported by NIH National Institute of Allergy and Infectious Diseases grant R01AI099210. I.B. and L.G. are supported by NIH grant AI 100190. F.Z. is supported by NIH grants 1R01-HG009761, 1R01-MH110049, and 1DP1-HL141201; HHMI; the New York Stem Cell, Allen, and Vallee foundations; the Tan-Yang Center at MIT; and J. and P. Poitras and R. Metcalfe. F.Z. is a New York Stem Cell Foundation–Robertson Investigator. M.L.N. is supported by the Sao Paulo Research Foundation (FAPESP; grant 13/21719-3) and is a Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq) Research Fellow. Publisher Copyright: 2017 {\textcopyright} The Authors.",
year = "2018",
month = apr,
day = "27",
doi = "10.1126/science.aas8836",
language = "English (US)",
volume = "360",
pages = "444--448",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6387",
}