Field-deployable viral diagnostics using CRISPR-Cas13

Cameron Myhrvold, Catherine A. Freije, Jonathan S. Gootenberg, Omar O. Abudayyeh, Hayden C. Metsky, Ann F. Durbin, Max J. Kellner, Amanda L. Tan, Lauren M. Paul, Leda A. Parham, Kimberly F. Garcia, Kayla G. Barnes, Bridget Chak, Adriano Mondini, Mauricio L. Nogueira, Sharon Isern, Scott F. Michael, Ivette Lorenzana, Nathan L. Yozwiak, Bronwyn L. MacInnisIrene Bosch, Lee Gehrke, Feng Zhang, Pardis C. Sabeti

Research output: Contribution to journalArticlepeer-review

373 Scopus citations

Abstract

Mitigating global infectious disease requires diagnostic tools that are sensitive, specific, and rapidly field deployable. In this study, we demonstrate that the Cas13-based SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) platform can detect Zika virus (ZIKV) and dengue virus (DENV) in patient samples at concentrations as low as 1 copy per microliter. We developed HUDSON (heating unextracted diagnostic samples to obliterate nucleases), a protocol that pairs with SHERLOCK for viral detection directly from bodily fluids, enabling instrument-free DENV detection directly from patient samples in <2 hours. We further demonstrate that SHERLOCK can distinguish the four DENV serotypes, as well as region-specific strains of ZIKV from the 2015–2016 pandemic. Finally, we report the rapid (<1 week) design and testing of instrument-free assays to detect clinically relevant viral single-nucleotide polymorphisms.

Original languageEnglish (US)
Pages (from-to)444-448
Number of pages5
JournalScience
Volume360
Issue number6387
DOIs
StatePublished - Apr 27 2018
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

Fingerprint

Dive into the research topics of 'Field-deployable viral diagnostics using CRISPR-Cas13'. Together they form a unique fingerprint.

Cite this