Femtosecond pump repump depletion to sort biological particles from background urban particles

V. Boutou; L. Guyon; C. Bonnet; M. Roth; H. Rabitz; F. Courvoisier; J. P. Wolf

doi:10.1117/12.689758

Femtosecond pump repump depletion to sort biological particles from background urban particles

V. Boutou, L. Guyon, C. Bonnet, M. Roth, H. Rabitz, F. Courvoisier, J. P. Wolf

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We investigated femtosecond pump-repump depletion excitation in biological fluorescent molecules (tryptophan and flavins) in solutions and in organic fluorescent interferants such as polycyclic hydrocarbons (naphthalene, diesel fuel). If the repump pulse induces in both flavins and Trp a depletion of the excited state, populated by the pump pulse, which leads to a drastic decrease of the fluorescence, such mechanism is ineffective in organic fluorescent interferents. The repump induced depletion is still observed for bacteria containing solutions. This opens interesting perspectives to discriminate biological from non-biological fluorescent particles in air.

Original language	English (US)
Title of host publication	Femtosecond Phenomena and Nonlinear Optics III
DOIs	https://doi.org/10.1117/12.689758
State	Published - 2006
Event	Femtosecond Phenomena and Nonlinear Optics III - Stockholm, Sweden Duration: Sep 11 2006 → Sep 12 2006

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	6400
ISSN (Print)	0277-786X

Other

Other	Femtosecond Phenomena and Nonlinear Optics III
Country/Territory	Sweden
City	Stockholm
Period	9/11/06 → 9/12/06

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Keywords

Aerosol particles discrimination
Bio-molecules ultrafast spectroscopy
Biological intrinsic fluorophore
Femtosecond fluorescence depletion
Real-time particle detection

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10.1117/12.689758

Cite this

Boutou, V., Guyon, L., Bonnet, C., Roth, M., Rabitz, H., Courvoisier, F., & Wolf, J. P. (2006). Femtosecond pump repump depletion to sort biological particles from background urban particles. In Femtosecond Phenomena and Nonlinear Optics III Article 64000N (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6400). https://doi.org/10.1117/12.689758

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title = "Femtosecond pump repump depletion to sort biological particles from background urban particles",

abstract = "We investigated femtosecond pump-repump depletion excitation in biological fluorescent molecules (tryptophan and flavins) in solutions and in organic fluorescent interferants such as polycyclic hydrocarbons (naphthalene, diesel fuel). If the repump pulse induces in both flavins and Trp a depletion of the excited state, populated by the pump pulse, which leads to a drastic decrease of the fluorescence, such mechanism is ineffective in organic fluorescent interferents. The repump induced depletion is still observed for bacteria containing solutions. This opens interesting perspectives to discriminate biological from non-biological fluorescent particles in air.",

keywords = "Aerosol particles discrimination, Bio-molecules ultrafast spectroscopy, Biological intrinsic fluorophore, Femtosecond fluorescence depletion, Real-time particle detection",

author = "V. Boutou and L. Guyon and C. Bonnet and M. Roth and H. Rabitz and F. Courvoisier and Wolf, {J. P.}",

year = "2006",

doi = "10.1117/12.689758",

language = "English (US)",

isbn = "0819464988",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

booktitle = "Femtosecond Phenomena and Nonlinear Optics III",

note = "Femtosecond Phenomena and Nonlinear Optics III ; Conference date: 11-09-2006 Through 12-09-2006",

}

Boutou, V, Guyon, L, Bonnet, C, Roth, M, Rabitz, H, Courvoisier, F & Wolf, JP 2006, Femtosecond pump repump depletion to sort biological particles from background urban particles. in Femtosecond Phenomena and Nonlinear Optics III., 64000N, Proceedings of SPIE - The International Society for Optical Engineering, vol. 6400, Femtosecond Phenomena and Nonlinear Optics III, Stockholm, Sweden, 9/11/06. https://doi.org/10.1117/12.689758

Femtosecond pump repump depletion to sort biological particles from background urban particles. / Boutou, V.; Guyon, L.; Bonnet, C. et al.
Femtosecond Phenomena and Nonlinear Optics III. 2006. 64000N (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6400).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Femtosecond pump repump depletion to sort biological particles from background urban particles

AU - Boutou, V.

AU - Guyon, L.

AU - Bonnet, C.

AU - Roth, M.

AU - Rabitz, H.

AU - Courvoisier, F.

AU - Wolf, J. P.

PY - 2006

Y1 - 2006

N2 - We investigated femtosecond pump-repump depletion excitation in biological fluorescent molecules (tryptophan and flavins) in solutions and in organic fluorescent interferants such as polycyclic hydrocarbons (naphthalene, diesel fuel). If the repump pulse induces in both flavins and Trp a depletion of the excited state, populated by the pump pulse, which leads to a drastic decrease of the fluorescence, such mechanism is ineffective in organic fluorescent interferents. The repump induced depletion is still observed for bacteria containing solutions. This opens interesting perspectives to discriminate biological from non-biological fluorescent particles in air.

AB - We investigated femtosecond pump-repump depletion excitation in biological fluorescent molecules (tryptophan and flavins) in solutions and in organic fluorescent interferants such as polycyclic hydrocarbons (naphthalene, diesel fuel). If the repump pulse induces in both flavins and Trp a depletion of the excited state, populated by the pump pulse, which leads to a drastic decrease of the fluorescence, such mechanism is ineffective in organic fluorescent interferents. The repump induced depletion is still observed for bacteria containing solutions. This opens interesting perspectives to discriminate biological from non-biological fluorescent particles in air.

KW - Aerosol particles discrimination

KW - Bio-molecules ultrafast spectroscopy

KW - Biological intrinsic fluorophore

KW - Femtosecond fluorescence depletion

KW - Real-time particle detection

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M3 - Conference contribution

AN - SCOPUS:33846250405

SN - 0819464988

SN - 9780819464989

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Femtosecond Phenomena and Nonlinear Optics III

T2 - Femtosecond Phenomena and Nonlinear Optics III

Y2 - 11 September 2006 through 12 September 2006

ER -

Femtosecond pump repump depletion to sort biological particles from background urban particles

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