Femtosecond pump repump depletion to sort biological particles from background urban particles

V. Boutou, L. Guyon, C. Bonnet, M. Roth, H. Rabitz, F. Courvoisier, J. P. Wolf

Research output: Chapter in Book/Report/Conference proceedingConference contribution


We investigated femtosecond pump-repump depletion excitation in biological fluorescent molecules (tryptophan and flavins) in solutions and in organic fluorescent interferants such as polycyclic hydrocarbons (naphthalene, diesel fuel). If the repump pulse induces in both flavins and Trp a depletion of the excited state, populated by the pump pulse, which leads to a drastic decrease of the fluorescence, such mechanism is ineffective in organic fluorescent interferents. The repump induced depletion is still observed for bacteria containing solutions. This opens interesting perspectives to discriminate biological from non-biological fluorescent particles in air.

Original languageEnglish (US)
Title of host publicationFemtosecond Phenomena and Nonlinear Optics III
StatePublished - 2006
EventFemtosecond Phenomena and Nonlinear Optics III - Stockholm, Sweden
Duration: Sep 11 2006Sep 12 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X


OtherFemtosecond Phenomena and Nonlinear Optics III

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


  • Aerosol particles discrimination
  • Bio-molecules ultrafast spectroscopy
  • Biological intrinsic fluorophore
  • Femtosecond fluorescence depletion
  • Real-time particle detection


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