Femtosecond laser pulses distinguish bacteria from background urban aerosols

Fraņois Courvoisier; V́ronique Boutou; Vanessa Wood; Andreas Bartelt; Matthias Roth; Herschel Rabitz; Jean Pierre Wolf

doi:10.1063/1.2007870

Femtosecond laser pulses distinguish bacteria from background urban aerosols

Fraņois Courvoisier, V́ronique Boutou, Vanessa Wood, Andreas Bartelt, Matthias Roth, Herschel Rabitz, Jean Pierre Wolf

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The fluorescence from living bacteria (Bacillus subtilis, Enteroccocus and Escherichia coli), induced by a ultrashort 270 nm pump pulse is depleted up to 50% by an optically delayed ultrafast 810 nm probe pulse in a pump-probe arrangement. The fast (subpicosecond) fluorescence decrease occurs for a pump-probe delay of Δt>2 ps. Depletion is also observed for tryptophan in water in contrast with organic cyclic molecules such as naphtalene or diesel fuel, despite similar absorption and fluorescence spectra. This remarkable difference allows us to propose a new remote sensing method able to efficiently discriminate organic from biological aerosols in highly populated urban areas.

Original language	English (US)
Article number	063901
Journal	Applied Physics Letters
Volume	87
Issue number	6
DOIs	https://doi.org/10.1063/1.2007870
State	Published - 2005

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

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10.1063/1.2007870

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title = "Femtosecond laser pulses distinguish bacteria from background urban aerosols",

abstract = "The fluorescence from living bacteria (Bacillus subtilis, Enteroccocus and Escherichia coli), induced by a ultrashort 270 nm pump pulse is depleted up to 50% by an optically delayed ultrafast 810 nm probe pulse in a pump-probe arrangement. The fast (subpicosecond) fluorescence decrease occurs for a pump-probe delay of Δt>2 ps. Depletion is also observed for tryptophan in water in contrast with organic cyclic molecules such as naphtalene or diesel fuel, despite similar absorption and fluorescence spectra. This remarkable difference allows us to propose a new remote sensing method able to efficiently discriminate organic from biological aerosols in highly populated urban areas.",

author = "Fraņois Courvoisier and {\'V}ronique Boutou and Vanessa Wood and Andreas Bartelt and Matthias Roth and Herschel Rabitz and Wolf, {Jean Pierre}",

note = "Funding Information: J.-P.W. thanks P. Callis, S. C. Hill and P. Hargis for fruitful discussions. H.R. acknowledges the support of the (U.S.) Army Research Office and the DoD Multidisciplinary University Research Initiative Program. J.-P.W. acknowledges NATO{\textquoteright}s Grant No. SSTCLG977928. F.C., V.B., and J.-P.W. aknowledge DGA{\textquoteright}s Grant No. 036000036004707501.",

year = "2005",

doi = "10.1063/1.2007870",

language = "English (US)",

volume = "87",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics Publising LLC",

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T1 - Femtosecond laser pulses distinguish bacteria from background urban aerosols

AU - Courvoisier, Fraņois

AU - Boutou, V́ronique

AU - Wood, Vanessa

AU - Bartelt, Andreas

AU - Roth, Matthias

AU - Rabitz, Herschel

AU - Wolf, Jean Pierre

N1 - Funding Information: J.-P.W. thanks P. Callis, S. C. Hill and P. Hargis for fruitful discussions. H.R. acknowledges the support of the (U.S.) Army Research Office and the DoD Multidisciplinary University Research Initiative Program. J.-P.W. acknowledges NATO’s Grant No. SSTCLG977928. F.C., V.B., and J.-P.W. aknowledge DGA’s Grant No. 036000036004707501.

PY - 2005

Y1 - 2005

N2 - The fluorescence from living bacteria (Bacillus subtilis, Enteroccocus and Escherichia coli), induced by a ultrashort 270 nm pump pulse is depleted up to 50% by an optically delayed ultrafast 810 nm probe pulse in a pump-probe arrangement. The fast (subpicosecond) fluorescence decrease occurs for a pump-probe delay of Δt>2 ps. Depletion is also observed for tryptophan in water in contrast with organic cyclic molecules such as naphtalene or diesel fuel, despite similar absorption and fluorescence spectra. This remarkable difference allows us to propose a new remote sensing method able to efficiently discriminate organic from biological aerosols in highly populated urban areas.

AB - The fluorescence from living bacteria (Bacillus subtilis, Enteroccocus and Escherichia coli), induced by a ultrashort 270 nm pump pulse is depleted up to 50% by an optically delayed ultrafast 810 nm probe pulse in a pump-probe arrangement. The fast (subpicosecond) fluorescence decrease occurs for a pump-probe delay of Δt>2 ps. Depletion is also observed for tryptophan in water in contrast with organic cyclic molecules such as naphtalene or diesel fuel, despite similar absorption and fluorescence spectra. This remarkable difference allows us to propose a new remote sensing method able to efficiently discriminate organic from biological aerosols in highly populated urban areas.

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DO - 10.1063/1.2007870

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JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 6

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ER -

Femtosecond laser pulses distinguish bacteria from background urban aerosols

Abstract

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