Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels

Ruoming Peng; Chao Wang; Liangcheng Zhou; Qi Zhang; Weihua Zhang; Stephen Y. Chou

Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels

Ruoming Peng, Chao Wang, Liangcheng Zhou, Qi Zhang, Weihua Zhang, Stephen Y. Chou

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We report fabrication and performances of a new immunoassay device, which integrates a novel nanoplasmonic sensor array inside a microfluidic channel. The new assay has demonstrated 106 fold detection limit enhancement of a model direct Protein A immunoassay over glass reference (from 2nM to 850 aM, i.e. 120 ng/ml to 50 fg/ml) and 6 fold of incubation time reduction (2 hours to 20 min) compared to conventional 96-well plate immunoassay.

Original language	English (US)
Title of host publication	Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Publisher	Chemical and Biological Microsystems Society
Pages	266-268
Number of pages	3
ISBN (Print)	9780979806452
State	Published - Jan 1 2012
Event	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan Duration: Oct 28 2012 → Nov 1 2012

Other

Other	16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Country/Territory	Japan
City	Okinawa
Period	10/28/12 → 11/1/12

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Peng, R., Wang, C., Zhou, L., Zhang, Q., Zhang, W., & Chou, S. Y. (2012). Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 266-268). Chemical and Biological Microsystems Society.

Peng, Ruoming ; Wang, Chao ; Zhou, Liangcheng et al. / Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 266-268

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title = "Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels",

abstract = "We report fabrication and performances of a new immunoassay device, which integrates a novel nanoplasmonic sensor array inside a microfluidic channel. The new assay has demonstrated 106 fold detection limit enhancement of a model direct Protein A immunoassay over glass reference (from 2nM to 850 aM, i.e. 120 ng/ml to 50 fg/ml) and 6 fold of incubation time reduction (2 hours to 20 min) compared to conventional 96-well plate immunoassay.",

author = "Ruoming Peng and Chao Wang and Liangcheng Zhou and Qi Zhang and Weihua Zhang and Chou, {Stephen Y.}",

year = "2012",

month = jan,

day = "1",

language = "English (US)",

isbn = "9780979806452",

pages = "266--268",

booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",

publisher = "Chemical and Biological Microsystems Society",

note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",

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Peng, R, Wang, C, Zhou, L, Zhang, Q, Zhang, W & Chou, SY 2012, Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 266-268, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 10/28/12.

Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels. / Peng, Ruoming; Wang, Chao; Zhou, Liangcheng et al.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 266-268.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels

AU - Peng, Ruoming

AU - Wang, Chao

AU - Zhou, Liangcheng

AU - Zhang, Qi

AU - Zhang, Weihua

AU - Chou, Stephen Y.

PY - 2012/1/1

Y1 - 2012/1/1

N2 - We report fabrication and performances of a new immunoassay device, which integrates a novel nanoplasmonic sensor array inside a microfluidic channel. The new assay has demonstrated 106 fold detection limit enhancement of a model direct Protein A immunoassay over glass reference (from 2nM to 850 aM, i.e. 120 ng/ml to 50 fg/ml) and 6 fold of incubation time reduction (2 hours to 20 min) compared to conventional 96-well plate immunoassay.

AB - We report fabrication and performances of a new immunoassay device, which integrates a novel nanoplasmonic sensor array inside a microfluidic channel. The new assay has demonstrated 106 fold detection limit enhancement of a model direct Protein A immunoassay over glass reference (from 2nM to 850 aM, i.e. 120 ng/ml to 50 fg/ml) and 6 fold of incubation time reduction (2 hours to 20 min) compared to conventional 96-well plate immunoassay.

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M3 - Conference contribution

AN - SCOPUS:84901812009

SN - 9780979806452

SP - 266

EP - 268

BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

PB - Chemical and Biological Microsystems Society

T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Y2 - 28 October 2012 through 1 November 2012

ER -

Peng R, Wang C, Zhou L, Zhang Q, Zhang W, Chou SY. Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 266-268

Fabrication and demonstration of ultra-sensitive and fast fluorescence immunoassay using novel nanoplasmonic sensor inside microfluidic channels

Abstract

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All Science Journal Classification (ASJC) codes

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