Extended field-of-view and increased-signal 3D holographic illumination with time-division multiplexing

Samuel J. Yang; William E. Allen; Isaac Kauvar; Aaron S. Andalman; Noah P. Young; Christina K. Kim; James H. Marshel; Gordon Wetzstein; Karl Deisseroth

doi:10.1364/OE.23.032573

Extended field-of-view and increased-signal 3D holographic illumination with time-division multiplexing

Samuel J. Yang, William E. Allen, Isaac Kauvar, Aaron S. Andalman, Noah P. Young, Christina K. Kim, James H. Marshel, Gordon Wetzstein, Karl Deisseroth

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Phase spatial light modulators (SLMs) are widely used for generating multifocal three-dimensional (3D) illumination patterns, but these are limited to a field of view constrained by the pixel count or size of the SLM. Further, with two-photon SLM-based excitation, increasing the number of focal spots penalizes the total signal linearly - requiring more laser power than is available or can be tolerated by the sample. Here we analyze and demonstrate a method of using galvanometer mirrors to time-sequentially reposition multiple 3D holograms, both extending the field of view and increasing the total time-averaged two-photon signal. We apply our approach to 3D two-photon in vivo neuronal calcium imaging.

Original language	English (US)
Pages (from-to)	32573-32581
Number of pages	9
Journal	Optics Express
Volume	23
Issue number	25
DOIs	https://doi.org/10.1364/OE.23.032573
State	Published - Dec 14 2015
Externally published	Yes

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics

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10.1364/OE.23.032573

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title = "Extended field-of-view and increased-signal 3D holographic illumination with time-division multiplexing",

abstract = "Phase spatial light modulators (SLMs) are widely used for generating multifocal three-dimensional (3D) illumination patterns, but these are limited to a field of view constrained by the pixel count or size of the SLM. Further, with two-photon SLM-based excitation, increasing the number of focal spots penalizes the total signal linearly - requiring more laser power than is available or can be tolerated by the sample. Here we analyze and demonstrate a method of using galvanometer mirrors to time-sequentially reposition multiple 3D holograms, both extending the field of view and increasing the total time-averaged two-photon signal. We apply our approach to 3D two-photon in vivo neuronal calcium imaging.",

author = "Yang, \{Samuel J.\} and Allen, \{William E.\} and Isaac Kauvar and Andalman, \{Aaron S.\} and Young, \{Noah P.\} and Kim, \{Christina K.\} and Marshel, \{James H.\} and Gordon Wetzstein and Karl Deisseroth",

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doi = "10.1364/OE.23.032573",

language = "English (US)",

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journal = "Optics Express",

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TY - JOUR

T1 - Extended field-of-view and increased-signal 3D holographic illumination with time-division multiplexing

AU - Yang, Samuel J.

AU - Allen, William E.

AU - Kauvar, Isaac

AU - Andalman, Aaron S.

AU - Young, Noah P.

AU - Kim, Christina K.

AU - Marshel, James H.

AU - Wetzstein, Gordon

AU - Deisseroth, Karl

PY - 2015/12/14

Y1 - 2015/12/14

N2 - Phase spatial light modulators (SLMs) are widely used for generating multifocal three-dimensional (3D) illumination patterns, but these are limited to a field of view constrained by the pixel count or size of the SLM. Further, with two-photon SLM-based excitation, increasing the number of focal spots penalizes the total signal linearly - requiring more laser power than is available or can be tolerated by the sample. Here we analyze and demonstrate a method of using galvanometer mirrors to time-sequentially reposition multiple 3D holograms, both extending the field of view and increasing the total time-averaged two-photon signal. We apply our approach to 3D two-photon in vivo neuronal calcium imaging.

AB - Phase spatial light modulators (SLMs) are widely used for generating multifocal three-dimensional (3D) illumination patterns, but these are limited to a field of view constrained by the pixel count or size of the SLM. Further, with two-photon SLM-based excitation, increasing the number of focal spots penalizes the total signal linearly - requiring more laser power than is available or can be tolerated by the sample. Here we analyze and demonstrate a method of using galvanometer mirrors to time-sequentially reposition multiple 3D holograms, both extending the field of view and increasing the total time-averaged two-photon signal. We apply our approach to 3D two-photon in vivo neuronal calcium imaging.

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Extended field-of-view and increased-signal 3D holographic illumination with time-division multiplexing

Abstract

All Science Journal Classification (ASJC) codes

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