Exploring the Förster limit in a small FRET pair

Yaser R. Khan; Tieneke E. Dykstra; Gregory D. Scholes

doi:10.1016/j.cplett.2008.07.023

Exploring the Förster limit in a small FRET pair

Yaser R. Khan, Tieneke E. Dykstra, Gregory D. Scholes

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

The accuracy of Förster theory for measuring short donor-acceptor distances in a small chromophore pair is examined. Small chromophores are much better suited for measuring short distances relative to large chormophores. Nonetheless, it is found that the Förster expression introduces errors for distances below 20 Å, overestimating for some orientations and underestimating for others. Fortuitous cancellation due to orientation disorder in experimental investigations may result in better agreement with Förster rate. Not only the size of the molecule, but more significantly, the spread of transition dipole density is found to be a limiting factor.

Original language	English (US)
Pages (from-to)	305-309
Number of pages	5
Journal	Chemical Physics Letters
Volume	461
Issue number	4-6
DOIs	https://doi.org/10.1016/j.cplett.2008.07.023
State	Published - Aug 20 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Physics and Astronomy
Physical and Theoretical Chemistry

Access to Document

10.1016/j.cplett.2008.07.023

Cite this

@article{7cfaf77f958f413b8f1eb5a4b0350b02,

title = "Exploring the F{\"o}rster limit in a small FRET pair",

abstract = "The accuracy of F{\"o}rster theory for measuring short donor-acceptor distances in a small chromophore pair is examined. Small chromophores are much better suited for measuring short distances relative to large chormophores. Nonetheless, it is found that the F{\"o}rster expression introduces errors for distances below 20 {\AA}, overestimating for some orientations and underestimating for others. Fortuitous cancellation due to orientation disorder in experimental investigations may result in better agreement with F{\"o}rster rate. Not only the size of the molecule, but more significantly, the spread of transition dipole density is found to be a limiting factor.",

author = "Khan, {Yaser R.} and Dykstra, {Tieneke E.} and Scholes, {Gregory D.}",

note = "Funding Information: The Natural Sciences and Engineering Research Council of Canada are gratefully acknowledged for support of this research. This work was made possible by the facilities of the Shared Hierarchical Academic Research Computing Network (SHARCNET: www.sharcnet.ca ). GDS acknowledges the support of an NSERC E.W.R. Steacie Memorial Fellowship.",

year = "2008",

month = aug,

day = "20",

doi = "10.1016/j.cplett.2008.07.023",

language = "English (US)",

volume = "461",

pages = "305--309",

journal = "Chemical Physics Letters",

issn = "0009-2614",

publisher = "Elsevier B.V.",

number = "4-6",

}

TY - JOUR

T1 - Exploring the Förster limit in a small FRET pair

AU - Khan, Yaser R.

AU - Dykstra, Tieneke E.

AU - Scholes, Gregory D.

N1 - Funding Information: The Natural Sciences and Engineering Research Council of Canada are gratefully acknowledged for support of this research. This work was made possible by the facilities of the Shared Hierarchical Academic Research Computing Network (SHARCNET: www.sharcnet.ca ). GDS acknowledges the support of an NSERC E.W.R. Steacie Memorial Fellowship.

PY - 2008/8/20

Y1 - 2008/8/20

N2 - The accuracy of Förster theory for measuring short donor-acceptor distances in a small chromophore pair is examined. Small chromophores are much better suited for measuring short distances relative to large chormophores. Nonetheless, it is found that the Förster expression introduces errors for distances below 20 Å, overestimating for some orientations and underestimating for others. Fortuitous cancellation due to orientation disorder in experimental investigations may result in better agreement with Förster rate. Not only the size of the molecule, but more significantly, the spread of transition dipole density is found to be a limiting factor.

AB - The accuracy of Förster theory for measuring short donor-acceptor distances in a small chromophore pair is examined. Small chromophores are much better suited for measuring short distances relative to large chormophores. Nonetheless, it is found that the Förster expression introduces errors for distances below 20 Å, overestimating for some orientations and underestimating for others. Fortuitous cancellation due to orientation disorder in experimental investigations may result in better agreement with Förster rate. Not only the size of the molecule, but more significantly, the spread of transition dipole density is found to be a limiting factor.

UR - http://www.scopus.com/inward/record.url?scp=48749094172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48749094172&partnerID=8YFLogxK

U2 - 10.1016/j.cplett.2008.07.023

DO - 10.1016/j.cplett.2008.07.023

M3 - Article

AN - SCOPUS:48749094172

SN - 0009-2614

VL - 461

SP - 305

EP - 309

JO - Chemical Physics Letters

JF - Chemical Physics Letters

IS - 4-6

ER -

Exploring the Förster limit in a small FRET pair

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this