Expansion and length-dependent fragility of CTG repeats in yeast

Catherine H. Freudenreich, Sara M. Kantrow, Virginia A. Zakian

Research output: Contribution to journalArticlepeer-review

367 Scopus citations


Expansion of DNA trinucleotide repeats (TNRs) is the causative mutation in a growing number of human genetic diseases. Large expansions of a CTG tract were obtained and shown by genetic and physical assays to be length- dependent sites of chromosome breakage in Saccharomyces cerevisiae. Deletion of RAD27, which encodes a nuclease involved in Okazaki fragment processing, caused length-dependent destabilization of CTG tracts and a substantial increase in expansion frequency. The genetic assay described here can be used to evaluate other factors that induce TNR expansion or chromosome fragility in humans.

Original languageEnglish (US)
Pages (from-to)853-856
Number of pages4
Issue number5352
StatePublished - Feb 6 1998

All Science Journal Classification (ASJC) codes

  • General


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