Expansion and length-dependent fragility of CTG repeats in yeast

Catherine H. Freudenreich, Sara M. Kantrow, Virginia Araxie Zakian

Research output: Contribution to journalArticle

348 Scopus citations

Abstract

Expansion of DNA trinucleotide repeats (TNRs) is the causative mutation in a growing number of human genetic diseases. Large expansions of a CTG tract were obtained and shown by genetic and physical assays to be length- dependent sites of chromosome breakage in Saccharomyces cerevisiae. Deletion of RAD27, which encodes a nuclease involved in Okazaki fragment processing, caused length-dependent destabilization of CTG tracts and a substantial increase in expansion frequency. The genetic assay described here can be used to evaluate other factors that induce TNR expansion or chromosome fragility in humans.

Original languageEnglish (US)
Pages (from-to)853-856
Number of pages4
JournalScience
Volume279
Issue number5352
DOIs
StatePublished - Feb 6 1998

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • General

Fingerprint Dive into the research topics of 'Expansion and length-dependent fragility of CTG repeats in yeast'. Together they form a unique fingerprint.

  • Cite this

    Freudenreich, C. H., Kantrow, S. M., & Zakian, V. A. (1998). Expansion and length-dependent fragility of CTG repeats in yeast. Science, 279(5352), 853-856. https://doi.org/10.1126/science.279.5352.853