Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

Frank J. Piscotta, Jeffery M. Tharp, Wenshe R. Liu, A. James Link

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Using the amber suppression approach, four noncanonical amino acids (ncAAs) were used to replace existing amino acids at four positions in lasso peptide microcin J25 (MccJ25). The lasso peptide biosynthesis enzymes tolerated all four ncAAs and produced antibiotics with efficacy equivalent to wild-type in some cases. Given the rapid expansion of the genetically encoded ncAA pool, this study is the first to demonstrate an expedient method to significantly increase the chemical diversity of lasso peptides. This journal is

Original languageEnglish (US)
Pages (from-to)409-412
Number of pages4
JournalChemical Communications
Volume51
Issue number2
DOIs
StatePublished - Jan 7 2015

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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