Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

Frank J. Piscotta; Jeffery M. Tharp; Wenshe R. Liu; A. James Link

doi:10.1039/c4cc07778d

Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

Frank J. Piscotta, Jeffery M. Tharp, Wenshe R. Liu, A. James Link

Research output: Contribution to journal › Article › peer-review

59 Scopus citations

Abstract

Using the amber suppression approach, four noncanonical amino acids (ncAAs) were used to replace existing amino acids at four positions in lasso peptide microcin J25 (MccJ25). The lasso peptide biosynthesis enzymes tolerated all four ncAAs and produced antibiotics with efficacy equivalent to wild-type in some cases. Given the rapid expansion of the genetically encoded ncAA pool, this study is the first to demonstrate an expedient method to significantly increase the chemical diversity of lasso peptides.

Original language	English (US)
Pages (from-to)	409-412
Number of pages	4
Journal	Chemical Communications
Volume	51
Issue number	2
DOIs	https://doi.org/10.1039/c4cc07778d
State	Published - Jan 7 2015

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
General Chemistry
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Catalysis

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abstract = "Using the amber suppression approach, four noncanonical amino acids (ncAAs) were used to replace existing amino acids at four positions in lasso peptide microcin J25 (MccJ25). The lasso peptide biosynthesis enzymes tolerated all four ncAAs and produced antibiotics with efficacy equivalent to wild-type in some cases. Given the rapid expansion of the genetically encoded ncAA pool, this study is the first to demonstrate an expedient method to significantly increase the chemical diversity of lasso peptides.",

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AU - Tharp, Jeffery M.

AU - Liu, Wenshe R.

AU - Link, A. James

N1 - Publisher Copyright: © The Royal Society of Chemistry.

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AB - Using the amber suppression approach, four noncanonical amino acids (ncAAs) were used to replace existing amino acids at four positions in lasso peptide microcin J25 (MccJ25). The lasso peptide biosynthesis enzymes tolerated all four ncAAs and produced antibiotics with efficacy equivalent to wild-type in some cases. Given the rapid expansion of the genetically encoded ncAA pool, this study is the first to demonstrate an expedient method to significantly increase the chemical diversity of lasso peptides.

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Expanding the chemical diversity of lasso peptide MccJ25 with genetically encoded noncanonical amino acids

Abstract

All Science Journal Classification (ASJC) codes

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