Evolutionary origins of the vertebrate heart: Specification of the cardiac lineage in Ciona intestinalis

Brad Davidson, Michael Levine

Research output: Contribution to journalArticle

99 Scopus citations

Abstract

Here we exploit the extensive cell lineage information and stream-lined genome of the ascidian, Ciona intestinalis, to investigate heart development in a basal chordate. Several cardiac genes were analyzed, including the sole Ciona ortholog of the Drosophila tinman gene, and tissue-specific enhancers were isolated for some of the genes. Conserved sequence motifs within these enhancers facilitated the isolation of a heart enhancer for the Ciona Hand-like gene. Altogether, these studies provide a regulatory framework for the differentiation of the cardiac mesoderm, beginning at the 110-cell stage, and extending through the fusion of cardiac progenitors during tail elongation. The cardiac lineage shares a common origin with the germ line, and zygotic transcription is first detected in the heart progenitors only after its separation from the germ line at the 64-cell stage. We propose that germ-line determinants influence the specification of the cardiac mesoderm, both by inhibiting inductive signals required for the development of noncardiac mesoderm lineages, and by providing a localized source of Wnt-5 and other signals required for heart development. We discuss the possibility that the germ line also influences the specification of the vertebrate heart.

Original languageEnglish (US)
Pages (from-to)11469-11473
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number20
DOIs
StatePublished - Sep 30 2003

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'Evolutionary origins of the vertebrate heart: Specification of the cardiac lineage in Ciona intestinalis'. Together they form a unique fingerprint.

  • Cite this