TY - JOUR
T1 - Evidence for a new step in telomere maintenance
AU - Wellinger, Raymund J.
AU - Ethier, Katia
AU - Labrecque, Pascale
AU - Zakian, Virginia A.
N1 - Funding Information:
Correspondence should be addressed to R. J. W. We thank the members of the Zakian lab for their stimulating input in the course of these experiments and A. Taylor for help with the electron microscopy experiments. D. Gottschling and V. Schultz are acknowledged for reading the manuscript and for constructive criticisms. We would like to note that this project and some experiments were initiated while R. J. W. and V. A. Z. were affiliated with the Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Washington 98104. This research was supported by grants from the Canadian Medical Research Council (MT-12616) to R. J. W. and from the National Institutes of Health (GM26938) to V. A. Z.; R. J. W. is a Chercheur-Boursier JuniorII of the Fonds de la Recherche en Santé du Québec.
PY - 1996/5/3
Y1 - 1996/5/3
N2 - The strand of telomeric DNA that runs 5'-3' toward a chromosome end is typically G rich. Telomerase-generated G tails are expected at one end of individual DNA molecules. Saccharomyces telomeres acquire TG1-3 tails late in S phase. Moreover, the telomeres of linear plasmids can interact when the TG1-3 tails are present. Molecules that mimic the structures predicted for telomere replication intermediates were generated in vitro. These in vitro generated molecules formed telomere-telomere interactions similar to those on molecules isolated from yeast, but only if both ends that interacted had a TG1-3 tail. Moreover, TG1-3 tails were generated in vivo in cells lacking telomerase. These data suggest a new step in telomere maintenance, cell cycle-regulated degradation of the C1-3A strand, which can generate a potential substrate for telomerase and telomere-binding proteins at every telomere.
AB - The strand of telomeric DNA that runs 5'-3' toward a chromosome end is typically G rich. Telomerase-generated G tails are expected at one end of individual DNA molecules. Saccharomyces telomeres acquire TG1-3 tails late in S phase. Moreover, the telomeres of linear plasmids can interact when the TG1-3 tails are present. Molecules that mimic the structures predicted for telomere replication intermediates were generated in vitro. These in vitro generated molecules formed telomere-telomere interactions similar to those on molecules isolated from yeast, but only if both ends that interacted had a TG1-3 tail. Moreover, TG1-3 tails were generated in vivo in cells lacking telomerase. These data suggest a new step in telomere maintenance, cell cycle-regulated degradation of the C1-3A strand, which can generate a potential substrate for telomerase and telomere-binding proteins at every telomere.
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U2 - 10.1016/S0092-8674(00)81120-4
DO - 10.1016/S0092-8674(00)81120-4
M3 - Article
C2 - 8616897
AN - SCOPUS:0029953557
SN - 0092-8674
VL - 85
SP - 423
EP - 433
JO - Cell
JF - Cell
IS - 3
ER -