@article{3759f744c95d431c95463ecc4872163e,
title = "Evaluating totipotency using criteria of increasing stringency",
abstract = "Totipotency is the ability of a single cell to give rise to all of the differentiated cell types that build the conceptus, yet how to capture this property in vitro remains incompletely understood. Defining totipotency relies on a variety of assays of variable stringency. Here, we describe criteria to define totipotency. We explain how distinct criteria of increasing stringency can be used to judge totipotency by evaluating candidate totipotent cell types in mice, including early blastomeres and expanded or extended pluripotent stem cells. Our data challenge the notion that expanded or extended pluripotent states harbour increased totipotent potential relative to conventional embryonic stem cells under in vitro and in vivo conditions.",
author = "Eszter Posfai and Schell, {John Paul} and Adrian Janiszewski and Isidora Rovic and Alexander Murray and Brian Bradshaw and Tatsuya Yamakawa and Tine Pardon and {El Bakkali}, Mouna and Irene Talon and {De Geest}, Natalie and Pankaj Kumar and To, {San Kit} and Sophie Petropoulos and Andrea Jurisicova and Vincent Pasque and Fredrik Lanner and Janet Rossant",
note = "Funding Information: We acknowledge the contribution of the Model Production Core staff at the Center for Phenogenomics for technical support. Single-cell sequencing was performed at National Genomics Infrastructure in Stockholm at the Science for Life Laboratory (funded by the Knut and Alice Wallenberg Foundation and the Swedish Research Council) with assistance from SNIC/Uppsala Multidisciplinary Center for Advanced Computational Science with massively parallel sequencing and access to the UPPMAX computational infrastructure. Bulk RNA-seq was performed at the KU Leuven Genomics Core. We thank S. Aerts, S. Aibar, C. Davie and C. Flerin for discussions and support; and J. Wu for the C1-12 tdTomato-expressing D-EPSCs. This work was funded by CIHR (FDN-143334), Genome Canada and Ontario Genomics (OGI-099), Programme de bourses de chercheur-boursier FRQS Junior 1 (FRQS 268829, 280187), the Swedish Research Council, Ragnar S{\"o}derberg Foundation, Ming Wai Lau Center for Reparative Medicine, Center for Innovative Medicine, Wallenberg Academy Fellow, NSERC (2014-04497). Research in the Pasque laboratory is supported by The Research Foundation–Flanders (FWO; Odysseus Return Grant G0F7716N to V.P.; FWO grants G0C9320N and G0B4420N to V.P.), the KU Leuven Research Fund (BOFZAP starting grant StG/15/021BF to V.P. C1 grant C14/16/077 to V.P. and project financing) and FWO PhD fellowships to A.Janiszewski (1158318N), I.T. (1S72719N) and S.K.T. (1S75720N). Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive licence to Springer Nature Limited.",
year = "2021",
month = jan,
doi = "10.1038/s41556-020-00609-2",
language = "English (US)",
volume = "23",
pages = "49--60",
journal = "Nature Cell Biology",
issn = "1465-7392",
publisher = "Nature Publishing Group",
number = "1",
}