Est1p as a cell cycle-regulated activator of telomere-bound telomerase

Andrew K.P. Taggart, Shu Chun Teng, Virginia A. Zakian

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Abstract

In Saccharomyces cerevisiae, the telomerase components Est2p, TLC1 RNA, Est1p, and Est3p are thought to form a complex that acts late during chromosome replication (S phase) upon recruitment by Cdc13p, a telomeric DNA binding protein. Consistent with this model, we show that Est1p, Est2p, and Cdc13p are telomere-associated at this time. However, Est2p, but not Est1p, also binds telomeres before late S phase. The cdc13-2 allele has been proposed to be defective in recruitment, yet Est1p and Est2p telomere association persists in cdc13-2 cells. These findings suggest a model in which Est1p binds telomeres late in S phase and interacts with Cdc13p to convert inactive, telomere-bound Est2p to an active form.

Original languageEnglish (US)
Pages (from-to)1023-1026
Number of pages4
JournalScience
Volume297
Issue number5583
DOIs
StatePublished - Aug 9 2002

All Science Journal Classification (ASJC) codes

  • General

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    Taggart, A. K. P., Teng, S. C., & Zakian, V. A. (2002). Est1p as a cell cycle-regulated activator of telomere-bound telomerase. Science, 297(5583), 1023-1026. https://doi.org/10.1126/science.1074968