Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes

Ingo H. Gorr; Alexandra Reis; Dominik Boos; Martin Wühr; Suzanne Madgwick; Keith T. Jones; Olaf Stemmann

doi:10.1038/ncb1467

Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes

Ingo H. Gorr
, Alexandra Reis
, Dominik Boos
, Martin Wühr
, Suzanne Madgwick
, Keith T. Jones
, Olaf Stemmann

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.

Original language	English (US)
Pages (from-to)	1035-1037
Number of pages	3
Journal	Nature cell biology
Volume	8
Issue number	9
DOIs	https://doi.org/10.1038/ncb1467
State	Published - Sep 2006
Externally published	Yes

All Science Journal Classification (ASJC) codes

Cell Biology

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10.1038/ncb1467

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title = "Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes",

abstract = "Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.",

author = "Gorr, \{Ingo H.\} and Alexandra Reis and Dominik Boos and Martin W{\"u}hr and Suzanne Madgwick and Jones, \{Keith T.\} and Olaf Stemmann",

note = "Funding Information: We thank S. Jentsch for continuous support and A. Stra{\ss}er for technical assistance. This work was supported by grants from the Deutsche Forschungsgemeinschaft (DFG; Emmy-Noether-Program) to O.S. and from The Wellcome Trust (Project 075744) to K.T.J. O.S. also received a career development award (CDA) from the International Human Frontier Science Program Organization.",

year = "2006",

month = sep,

doi = "10.1038/ncb1467",

language = "English (US)",

volume = "8",

pages = "1035--1037",

journal = "Nature cell biology",

issn = "1465-7392",

publisher = "Nature Research",

number = "9",

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TY - JOUR

T1 - Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes

AU - Gorr, Ingo H.

AU - Reis, Alexandra

AU - Boos, Dominik

AU - Wühr, Martin

AU - Madgwick, Suzanne

AU - Jones, Keith T.

AU - Stemmann, Olaf

N1 - Funding Information: We thank S. Jentsch for continuous support and A. Straßer for technical assistance. This work was supported by grants from the Deutsche Forschungsgemeinschaft (DFG; Emmy-Noether-Program) to O.S. and from The Wellcome Trust (Project 075744) to K.T.J. O.S. also received a career development award (CDA) from the International Human Frontier Science Program Organization.

PY - 2006/9

Y1 - 2006/9

N2 - Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.

AB - Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.

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UR - https://www.scopus.com/pages/publications/33748206357#tab=citedBy

U2 - 10.1038/ncb1467

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EP - 1037

JO - Nature cell biology

JF - Nature cell biology

IS - 9

ER -

Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes

Abstract

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