Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes

Ingo H. Gorr, Alexandra Reis, Dominik Boos, Martin Wühr, Suzanne Madgwick, Keith T. Jones, Olaf Stemmann

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.

Original languageEnglish (US)
Pages (from-to)1035-1037
Number of pages3
JournalNature cell biology
Volume8
Issue number9
DOIs
StatePublished - Sep 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

Fingerprint Dive into the research topics of 'Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes'. Together they form a unique fingerprint.

  • Cite this

    Gorr, I. H., Reis, A., Boos, D., Wühr, M., Madgwick, S., Jones, K. T., & Stemmann, O. (2006). Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes. Nature cell biology, 8(9), 1035-1037. https://doi.org/10.1038/ncb1467