Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes

Ingo H. Gorr, Alexandra Reis, Dominik Boos, Martin Wühr, Suzanne Madgwick, Keith T. Jones, Olaf Stemmann

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase-Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.

Original languageEnglish (US)
Pages (from-to)1035-1037
Number of pages3
JournalNature cell biology
Issue number9
StatePublished - Sep 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology


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