EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genes

Frank D. Russo, Thomas Joseph Silhavy

Research output: Contribution to journalArticle

151 Scopus citations

Abstract

Osmoregulation of the bacterial porin genes ompF and ompC is controlled by a twocomponent regulatory system. EnvZ, the sensor component of this system, is capable both of phosphorylating and dephosphorylating OmpR, the effector component. Mutations were isolated in envZ that abolish the expression of both porin genes. These mutants appear to have lost the kinase activity of EnvZ while retaining their phosphatase activity, so that in their presence OmpR is completely unphosphorylated. The behavior of these mutants in haploid, and in diploid with other envZ alleles, is consistent with a model in which EnvZ mediates osmoregulation by controlling the concentration of a single species, OmpR-P.

Original languageEnglish (US)
Pages (from-to)567-580
Number of pages14
JournalJournal of Molecular Biology
Volume222
Issue number3
DOIs
StatePublished - Dec 5 1991

All Science Journal Classification (ASJC) codes

  • Molecular Biology

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