TY - JOUR
T1 - EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genes
AU - Russo, Frank D.
AU - Silhavy, Thomas Joseph
PY - 1991/12/5
Y1 - 1991/12/5
N2 - Osmoregulation of the bacterial porin genes ompF and ompC is controlled by a twocomponent regulatory system. EnvZ, the sensor component of this system, is capable both of phosphorylating and dephosphorylating OmpR, the effector component. Mutations were isolated in envZ that abolish the expression of both porin genes. These mutants appear to have lost the kinase activity of EnvZ while retaining their phosphatase activity, so that in their presence OmpR is completely unphosphorylated. The behavior of these mutants in haploid, and in diploid with other envZ alleles, is consistent with a model in which EnvZ mediates osmoregulation by controlling the concentration of a single species, OmpR-P.
AB - Osmoregulation of the bacterial porin genes ompF and ompC is controlled by a twocomponent regulatory system. EnvZ, the sensor component of this system, is capable both of phosphorylating and dephosphorylating OmpR, the effector component. Mutations were isolated in envZ that abolish the expression of both porin genes. These mutants appear to have lost the kinase activity of EnvZ while retaining their phosphatase activity, so that in their presence OmpR is completely unphosphorylated. The behavior of these mutants in haploid, and in diploid with other envZ alleles, is consistent with a model in which EnvZ mediates osmoregulation by controlling the concentration of a single species, OmpR-P.
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U2 - 10.1016/0022-2836(91)90497-T
DO - 10.1016/0022-2836(91)90497-T
M3 - Article
C2 - 1660927
AN - SCOPUS:0026333013
VL - 222
SP - 567
EP - 580
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
SN - 0022-2836
IS - 3
ER -