EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genes

Frank D. Russo; Thomas J. Silhavy

doi:10.1016/0022-2836(91)90497-T

EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genes

Frank D. Russo, Thomas J. Silhavy

Molecular Biology

Research output: Contribution to journal › Article › peer-review

157 Scopus citations

Abstract

Osmoregulation of the bacterial porin genes ompF and ompC is controlled by a twocomponent regulatory system. EnvZ, the sensor component of this system, is capable both of phosphorylating and dephosphorylating OmpR, the effector component. Mutations were isolated in envZ that abolish the expression of both porin genes. These mutants appear to have lost the kinase activity of EnvZ while retaining their phosphatase activity, so that in their presence OmpR is completely unphosphorylated. The behavior of these mutants in haploid, and in diploid with other envZ alleles, is consistent with a model in which EnvZ mediates osmoregulation by controlling the concentration of a single species, OmpR-P.

Original language	English (US)
Pages (from-to)	567-580
Number of pages	14
Journal	Journal of Molecular Biology
Volume	222
Issue number	3
DOIs	https://doi.org/10.1016/0022-2836(91)90497-T
State	Published - Dec 5 1991

All Science Journal Classification (ASJC) codes

Structural Biology
Molecular Biology

Keywords

osmoregulation
phosphorylation
repressor
signal transduction
transcriptional activator

Access to Document

10.1016/0022-2836(91)90497-T

Cite this

@article{6fbd18b87e584c9ab4aedada5aabe4f4,

title = "EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genes",

abstract = "Osmoregulation of the bacterial porin genes ompF and ompC is controlled by a twocomponent regulatory system. EnvZ, the sensor component of this system, is capable both of phosphorylating and dephosphorylating OmpR, the effector component. Mutations were isolated in envZ that abolish the expression of both porin genes. These mutants appear to have lost the kinase activity of EnvZ while retaining their phosphatase activity, so that in their presence OmpR is completely unphosphorylated. The behavior of these mutants in haploid, and in diploid with other envZ alleles, is consistent with a model in which EnvZ mediates osmoregulation by controlling the concentration of a single species, OmpR-P.",

keywords = "osmoregulation, phosphorylation, repressor, signal transduction, transcriptional activator",

author = "Russo, {Frank D.} and Silhavy, {Thomas J.}",

note = "Funding Information: We thank James Slauch, Michele Igo and Jeff Stock for helpful discussions. and the members of the Silhavy laboratory for critical reading of the manuscript. This work was supported by a grant (GM35791) from the Public Health Service to T.J.S. F.D.R. was support{\textquoteright}ed by a National Science Foundation Predoctoral Fellowship.",

year = "1991",

month = dec,

day = "5",

doi = "10.1016/0022-2836(91)90497-T",

language = "English (US)",

volume = "222",

pages = "567--580",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genes

AU - Russo, Frank D.

AU - Silhavy, Thomas J.

N1 - Funding Information: We thank James Slauch, Michele Igo and Jeff Stock for helpful discussions. and the members of the Silhavy laboratory for critical reading of the manuscript. This work was supported by a grant (GM35791) from the Public Health Service to T.J.S. F.D.R. was support’ed by a National Science Foundation Predoctoral Fellowship.

PY - 1991/12/5

Y1 - 1991/12/5

N2 - Osmoregulation of the bacterial porin genes ompF and ompC is controlled by a twocomponent regulatory system. EnvZ, the sensor component of this system, is capable both of phosphorylating and dephosphorylating OmpR, the effector component. Mutations were isolated in envZ that abolish the expression of both porin genes. These mutants appear to have lost the kinase activity of EnvZ while retaining their phosphatase activity, so that in their presence OmpR is completely unphosphorylated. The behavior of these mutants in haploid, and in diploid with other envZ alleles, is consistent with a model in which EnvZ mediates osmoregulation by controlling the concentration of a single species, OmpR-P.

AB - Osmoregulation of the bacterial porin genes ompF and ompC is controlled by a twocomponent regulatory system. EnvZ, the sensor component of this system, is capable both of phosphorylating and dephosphorylating OmpR, the effector component. Mutations were isolated in envZ that abolish the expression of both porin genes. These mutants appear to have lost the kinase activity of EnvZ while retaining their phosphatase activity, so that in their presence OmpR is completely unphosphorylated. The behavior of these mutants in haploid, and in diploid with other envZ alleles, is consistent with a model in which EnvZ mediates osmoregulation by controlling the concentration of a single species, OmpR-P.

KW - osmoregulation

KW - phosphorylation

KW - repressor

KW - signal transduction

KW - transcriptional activator

UR - http://www.scopus.com/inward/record.url?scp=0026333013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026333013&partnerID=8YFLogxK

U2 - 10.1016/0022-2836(91)90497-T

DO - 10.1016/0022-2836(91)90497-T

M3 - Article

C2 - 1660927

AN - SCOPUS:0026333013

VL - 222

SP - 567

EP - 580

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 3

ER -

EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genes

Abstract

All Science Journal Classification (ASJC) codes

Keywords

Access to Document

Other files and links

Fingerprint

Cite this