Enhancer competition between H19 and Igf2 does not mediate their imprinting

Jennifer V. Schmidt, John M. Levorse, Shirley M. Tilghman

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

The linked H19 and Igf2 genes on mouse distal chromosome 7 are subject to genomic imprinting. Competition between the promoters of the genes for transcription from shared enhancers has been proposed as an explanation for the coordinate expression and reciprocal imprinting of these two genes. To test this model, we have used Cre-loxP technology to generate in mice a conditional deletion of the H19 promoter and structural gene that leaves no transcription unit in the locus. Contrary to the prediction of enhancer competition we find that transcriptional activity from the H19 promoter is not required for the imprinted silencing of the Igf2 gene.

Original languageEnglish (US)
Pages (from-to)9733-9738
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number17
DOIs
StatePublished - Aug 17 1999

All Science Journal Classification (ASJC) codes

  • General

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