TY - JOUR
T1 - Enhancer competition between H19 and Igf2 does not mediate their imprinting
AU - Schmidt, Jennifer V.
AU - Levorse, John M.
AU - Tilghman, Shirley M.
PY - 1999/8/17
Y1 - 1999/8/17
N2 - The linked H19 and Igf2 genes on mouse distal chromosome 7 are subject to genomic imprinting. Competition between the promoters of the genes for transcription from shared enhancers has been proposed as an explanation for the coordinate expression and reciprocal imprinting of these two genes. To test this model, we have used Cre-loxP technology to generate in mice a conditional deletion of the H19 promoter and structural gene that leaves no transcription unit in the locus. Contrary to the prediction of enhancer competition we find that transcriptional activity from the H19 promoter is not required for the imprinted silencing of the Igf2 gene.
AB - The linked H19 and Igf2 genes on mouse distal chromosome 7 are subject to genomic imprinting. Competition between the promoters of the genes for transcription from shared enhancers has been proposed as an explanation for the coordinate expression and reciprocal imprinting of these two genes. To test this model, we have used Cre-loxP technology to generate in mice a conditional deletion of the H19 promoter and structural gene that leaves no transcription unit in the locus. Contrary to the prediction of enhancer competition we find that transcriptional activity from the H19 promoter is not required for the imprinted silencing of the Igf2 gene.
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U2 - 10.1073/pnas.96.17.9733
DO - 10.1073/pnas.96.17.9733
M3 - Article
C2 - 10449763
AN - SCOPUS:0033578336
SN - 0027-8424
VL - 96
SP - 9733
EP - 9738
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 17
ER -