Engineered tissues to quantify collective cell migration during morphogenesis

Sriram Manivannan, Jason P. Gleghorn, Celeste M. Nelson

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations


Renal development is a complex process involving the dynamic interplay of over 25 different cell types. One distinct step in this process is the formation of the ureteric tree, which develops from the repeated branching of the ureteric bud. During branching of the ureteric bud, cells migrate collectively in unison to form the complex structure of the tree. Here, we present a microlithography-based 3D culture model in which multiple identical kidney epithelial tissues are used to quantify collective cell migration during the process of branching morphogenesis.

Original languageEnglish (US)
Title of host publicationKidney Development
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages10
ISBN (Print)9781617798504
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology


  • Engineered tissue
  • Patterning
  • Persistence time


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