Engineered microenvironments to investigate cellular behavior

Srivatsan Raghavan, Celeste M. Nelson, Nathan J. Sniadecki, Emerson A. Lim, Christopher S. Chen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Numerous in vivo morphogenetic processes such as angiogenesis occur within a three-dimensional context, where both soluble growth factor-mediated and insoluble adhesive signals modulate cellular behaviors such as proliferation, differentiation, and apoptosis. Using microfabrication approaches to engineer the multidimensional cellular microenvironment, we are examining how these adhesive and growth factor cues interact and synergize to regulate dynamic cell signaling, gene expression, cell proliferation, and migration. Here, we demonstrate a novel microfabricated platform to culture cells within three-dimensional collagen gels that enables the spatial organization of single cells and multicellular aggregates. Our results suggest that in addition to the matrix environment, the spatiotemporal dynamics of a stimulus signal can influence intracellular signaling and subsequent cell behavior. These systems enable fundamental insights into how cells integrate mechanochemical signals from their environment in order to coordinate downstream functions.

Original languageEnglish (US)
Title of host publication28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
Number of pages1
DOIs
StatePublished - Dec 1 2006
Externally publishedYes
Event28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06 - New York, NY, United States
Duration: Aug 30 2006Sep 3 2006

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
ISSN (Print)0589-1019

Other

Other28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06
CountryUnited States
CityNew York, NY
Period8/30/069/3/06

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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  • Cite this

    Raghavan, S., Nelson, C. M., Sniadecki, N. J., Lim, E. A., & Chen, C. S. (2006). Engineered microenvironments to investigate cellular behavior. In 28th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'06 [4030471] (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings). https://doi.org/10.1109/IEMBS.2006.259301