@article{e4c6e37cd0024f34b9dfecccfb7ac9ce,
title = "Engagement of Arginine Finger to ATP Triggers Large Conformational Changes in NtrC1 AAA+ ATPase for Remodeling Bacterial RNA Polymerase",
abstract = "The NtrC-like AAA+ ATPases control virulence and other important bacterial activities through delivering mechanical work to σ54-RNA polymerase to activate transcription from σ54-dependent genes. We report the first crystal structure for such an ATPase, NtrC1 of Aquifex aeolicus, in which the catalytic arginine engages the γ-phosphate of ATP. Comparing the new structure with those previously known for apo and ADP-bound states supports a rigid-body displacement model that is consistent with large-scale conformational changes observed by low-resolution methods. First, the arginine finger induces rigid-body roll, extending surface loops above the plane of the ATPase ring to bind σ54. Second, ATP hydrolysis permits Pi release and retraction of the arginine with a reversed roll, remodeling σ54-RNAP. This model provides a fresh perspective on how ATPase subunits interact within the ring-ensemble to promote transcription, directing attention to structural changes on the arginine-finger side of an ATP-bound interface.",
author = "Baoyu Chen and Sysoeva, {Tatyana A.} and Saikat Chowdhury and Liang Guo and {De Carlo}, Sacha and Hanson, {Jeffrey A.} and Haw Yang and Nixon, {B. Tracy}",
note = "Funding Information: We thank Borries Demeler for facilitating our use of ATSAS software to perform ab initio modeling and averaging of SAXS data on the BCF cluster at the University of Texas Health Science Center at San Antonio, Neela Yennawar and Hemant Yennawar of the X-ray core facility at the Huck Institutes of Life Sciences, Penn State for help growing crystals and collecting initial diffraction data, and David Wemmer and Joseph Bachelor for help collecting and processing data on beamline 8.3.1 at the Advanced Light Source. The work was funded by NIH grant R01 GM069937-01A3 to B.T.N., and Use of the Advanced Photon Source was supported by the U.S. Department of Energy, Basic Energy Sciences, Office of Science, under contract no. W-31-109-ENG-38, and the Advanced Light Source is similarly supported under contract no. DE-AC02-05CH11231. BioCAT is a National Institutes of Health-supported Research Center, grant no. RR-08630. The X-ray core facility at Penn State was partially supported by NIN-NCRR grant 1S10RR023439-01. The authors declare that they have no competing financial interests. ",
year = "2010",
month = nov,
day = "10",
doi = "10.1016/j.str.2010.08.018",
language = "English (US)",
volume = "18",
pages = "1420--1430",
journal = "Structure",
issn = "0969-2126",
publisher = "Cell Press",
number = "11",
}