TY - JOUR
T1 - Emerging Chemistry Strategies for Engineering Native Chromatin
AU - David, Yael
AU - Muir, Tom W.
N1 - Funding Information:
Some of the work discussed in this Perspective was conducted in the Muir lab and was supported by U.S. National Institutes of Health grants R37-GM086868, R01-GM107047, and P01-CA196539. The David lab is supported by the CCSG core grant P30 CA008748 and the Josie Robertson Young Investigator Award.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/7/12
Y1 - 2017/7/12
N2 - Chromosomes present one of most challenging of all substrates for biochemical study. This is because genomic DNA is physically associated with an astonishing collection of nuclear factors, which serve to not only store the nucleic acid in a stable form, but also grant access to the information it encodes when needed. Understanding this complex molecular choreography is central to the field of epigenetics. One of the great challenges in this area is to move beyond correlative type information, which is now in abundant supply, to the point where we can truly connect the dots at the molecular level. Establishing such causal relationships requires precise manipulation of the covalent structure of chromatin. Tools for this purpose are currently in short supply, creating an opportunity that, as we will argue in this Perspective, is well suited to the sensibilities of the chemist.
AB - Chromosomes present one of most challenging of all substrates for biochemical study. This is because genomic DNA is physically associated with an astonishing collection of nuclear factors, which serve to not only store the nucleic acid in a stable form, but also grant access to the information it encodes when needed. Understanding this complex molecular choreography is central to the field of epigenetics. One of the great challenges in this area is to move beyond correlative type information, which is now in abundant supply, to the point where we can truly connect the dots at the molecular level. Establishing such causal relationships requires precise manipulation of the covalent structure of chromatin. Tools for this purpose are currently in short supply, creating an opportunity that, as we will argue in this Perspective, is well suited to the sensibilities of the chemist.
UR - http://www.scopus.com/inward/record.url?scp=85024401452&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85024401452&partnerID=8YFLogxK
U2 - 10.1021/jacs.7b03430
DO - 10.1021/jacs.7b03430
M3 - Review article
C2 - 28635271
AN - SCOPUS:85024401452
SN - 0002-7863
VL - 139
SP - 9090
EP - 9096
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 27
ER -